Development and characterization of microsatellite markers for genetic analysis of Moerella iridescens Benson, 1842
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TECHNICAL NOTE
Development and characterization of microsatellite markers for genetic analysis of Moerella iridescens Benson, 1842 Xiaoying Li • Zhiguo Dong • Meizhen Wang • Manman Zhao • Yumei Chang • Hanchun Chen
Received: 31 July 2012 / Accepted: 6 August 2012 / Published online: 17 August 2012 Ó Springer Science+Business Media B.V. 2012
Abstract Moerella iridescens (Benson) is an economically important small-sized edible marine clam from the Changjiang River delta region of China. In this study, 13 polymorphic microsatellite loci were isolated and characterized in a wild population from the Cixi mud flats. The number of alleles varied between two and six, and the observed and expected heterozygosity at the population level ranged from 0.2000 to 0.8095 and 0.3243 to 0.5106, respectively. No single locus significantly deviated from Hardy–Weinberg equilibrium (P [ 0.01). All of the polymorphic markers would be useful for future genome mapping, genetic diversity and population assessment studies in M. iridescens. Keywords Moerella iridescens Microsatellite loci Genetic characterization Polymorphic molecular markers
Moerella iridescens (Benson 1842) is widely distributed throughout the Northwest Pacific, China, Korea and Japan. It is a high-valued marine mollusk species and its fauna (Xu 1997), morphological characteristics (Cai and Huang 1991; Ji et al. 2007) and environmental toxicology (Wang et al.
X. Li Z. Dong (&) M. Zhao Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, China e-mail: [email protected] M. Wang H. Chen Cixi Fisheries Institute, Cixi 315300, China Y. Chang Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
2007) have been extensively studied. However, the population genetic diversity and genetic structure of the species have rarely been analyzed with the exception of studies based on RAPD and ISSR techniques (Yu et al. 2009; Dong et al. 2010). Due to unsustainable exploitation and ecological deterioration of its environment, M. iridescens resources have been declining, and a better understanding of its population genetics has become increasingly urgent. Microsatellite DNA markers are the most popular and powerful molecular markers in population genetics and are used for the construction of genetic linkage maps (Liu and Cordes 2004). Microsatellites have become one of the most commonly used molecular markers in population and evolutionary biology research (Goldstein and Pollock 1997). To date, no study has used microsatellite markers to analyze the population structure of this species. In this study, we first reported the isolation and characterization of 13 microsatellite loci in M. iridescens. Genomic DNA was extracted from the mantle muscle of 30 M. iridescens individuals through the standard phenol– chloroform procedure (Sambrook et al. 1989). The genomic DNA was used to construct a partially enriched genomic library for GA and CA-motif microsatellite sequences
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