Development of 18 novel polymorphic microsatellite markers for the mysid crustacean Mesopodopsis orientalis
- PDF / 157,729 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 24 Downloads / 188 Views
MICROSATELLITE LETTERS
Development of 18 novel polymorphic microsatellite markers for the mysid crustacean Mesopodopsis orientalis Noriyuki Koizumi • Yukio Hanamura • Kazuya Nishida Atsushi Mori • Keiji Watabe • Takeshi Takemura • Alias Man • Faizul M. Kassim • Shinsuke Morioka
•
Received: 5 August 2014 / Accepted: 20 August 2014 / Published online: 4 September 2014 Springer Science+Business Media Dordrecht 2014
Abstract We have developed microsatellite DNA markers for Mesopodopsis orientalis (Tattersall 1908), a widely distributed mysid crustacean in shallow waters of the coastal and estuarine systems across India to Indonesia and the Philippines. A total of 18 novel polymorphic microsatellite markers were identified in 32 individuals from a sandy beach at Teluk Kumbar in Penang Island, Malaysia. The number of alleles per locus ranged from 3 to 17. The observed and expected heterozygosities varied from 0.031–1.000 to 0.092–0.938, respectively. These polymorphic markers could be available for future investiga-
Electronic supplementary material The online version of this article (doi:10.1007/s12686-014-0311-8) contains supplementary material, which is available to authorized users. N. Koizumi (&) A. Mori K. Watabe T. Takemura National Institute for Rural Engineering, Tsukuba, Ibaraki 305-8609, Japan e-mail: [email protected] Y. Hanamura National Research Institute of Fisheries Science, Yokohama, Kanagawa 236-8648, Japan K. Nishida National Research Institute of Far Seas Fisheries, Kanazawa, Yokohama, Kanagawa 236-8648, Japan A. Man Fisheries Research Institute, Kampung Acheh, 32000 Sitawan, Perak, Malaysia F. M. Kassim S. Morioka Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan
tions of genetic diversity and structure of M. orientalis populations. Keywords Crustacea Mysida Microsatellite Genetic diversity Estuarine system
Mesopodopsis orientalis (Tattersall 1908) is widely distributed in shallow waters from India to Indonesia and the Philippines. This species plays an important role in the coastal and estuarine food webs. This mysid is harvested for human consumption and/or often feeds fish in aquaculture fields (Hanamura et al. 2008, 2009; Mantiri et al. 2012). Furthermore, the mysid crustacean has good potential to eventually be developed into a model organism for various bio-assays due to its abundance and ease in culturing (Eusebio et al. 2010). Thus, information on the genetic diversity and structure of local populations of this mysid are of emerging concern. Microsatellite DNA markers have been developed herein to aid the genetic investigation of M. orientalis. Microsatellite enriched libraries were isolated using magnetic bead capture enrichment (Glenn and Schable 2005). Genomic DNA was extracted from individuals using an automated DNA isolation system (KURABO). Extracted DNA was digested with RsaI (New England Biolabs) and then ligated to SuperSNX linkers. Linkerligated DNA was hybridized to biotinylated oligonucleotides (CA)12 and (CT)
Data Loading...
