Development of hyaluronan-based membranes for the healing of intestinal surgical wounds: a preliminary study
- PDF / 6,320,443 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 46 Downloads / 178 Views
B I O M A T E R I A L S S Y N T H E S I S A N D CH A R A C T E R I S A T I O N Original Research
Development of hyaluronan-based membranes for the healing of intestinal surgical wounds: a preliminary study Francesca Scognamiglio1 Andrea Travan1 Rossana Bussani2 Massimiliano Borgogna1 Ivan Donati1 Joanna W. A. M. Bosmans3 Nicole D. Bouvy3 Eleonora Marsich4 ●
●
●
●
●
●
●
1234567890();,:
1234567890();,:
Received: 27 February 2019 / Accepted: 6 May 2019 / Published online: 24 May 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Implantable membranes based on alginate and hyaluronic acid (HA) were manufactured to obtain a rapidly resorbing pliable mesh for the in situ administration of HA to intestinal tissue. Morphological analyses of this interpenetrated matrix pointed out a homogeneous polymeric texture while degradation studies demonstrated that the material is able to dissolve in physiological solutions within few days. Biological studies in vitro showed that the membrane is biocompatible towards human dermal fibroblasts and that liquid extracts from the HA-containing membrane can stimulate wound healing. A preliminary in vivo biocompatibility study on rats showed that the membranes in direct contact with the intestine did not elicit any acute adverse reaction or immune response, while only a mild inflammatory reaction was noticed at the mesenteric or serosal region. Overall, these results appear to support the application of these polysaccharide-based materials for intestinal wound healing.
Graphical Abstract
These authors contributed equally: Francesca Scognamiglio, Andrea Travan * Francesca Scognamiglio [email protected] 1
Department of Life Sciences, University of Trieste, Trieste, Italy
2
Institute of Pathological Anatomy, University of Trieste, Trieste, Italy
3
Department of Surgery, Research Institute NUTRIM, Maastricht University Medical Centre, Maastricht, Netherlands
4
Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy
1 Introduction Hyaluronic acid (HA) is a linear polysaccharide widely employed in the biomedical field for its ability to regulate physiological events such as fibroblasts migration, differentiation and proliferation [1–4], making it a good candidate for the development of biomaterials that can stimulate wound healing of tissues. This property can be exploited in surgical interventions that require a fast closure of wounds, in particular in the case of internal organs in human body. Among these organs, intestine represents a challenging target, and the possibility to develop biomaterials
60
Page 2 of 9
supporting intestinal healing has been explored in literature [5, 6]. Indeed, surgical wounds on intestinal tissues are mostly associated to the treatment of cancer, which often requires the resection of the affected tract, followed by the suture of intestinal extremities (anastomosis). A safe closure of the anastomosis occurs when a proper regeneration of the intestinal tissue takes place [7].
Data Loading...
