Effect of Titanium Dioxide Nanoparticles (TiO 2 NPs) on Faba bean ( Vicia faba L.) and Induced Asynaptic Mutation: A Mei
- PDF / 2,388,057 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 63 Downloads / 186 Views
Effect of Titanium Dioxide Nanoparticles (TiO2 NPs) on Faba bean (Vicia faba L.) and Induced Asynaptic Mutation: A Meiotic Study Kalyan Singh Kushwah1 · Sapan Patel1 Received: 21 May 2019 / Accepted: 17 October 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract We have synthesized titanium dioxide (TiO2) nanoparticles (NPs) and studied the cytogenotoxic effect of the synthesized NPs on plants. The synthesized NPs were characterized by XRD, SEM and PSA. The XRD results showed the formation of crystalline TiO2 nanoparticles. The SEM analysis showed that the synthesized nanoparticles range from 60 to 300 nm. The effect of synthesized TiO2 nanoparticles on Vicia faba (2n = 12) was studied. The seeds of Vicia faba were treated with different concentrations (15, 30, 60, 120 and 240 mg/L, or 1.5, 3.0, 6.0, 12.0 and 24.0 mg/100 mL) of TiO2 nanoparticles. Seeds treated with the higher concentrations of TiO2 showed a change in the meiotic activity which causes a significant increase of chromosomal abnormalities in the reproductive parts of the plant. Different types of meiotic abnormalities, such as stickiness and the separation of univalent and bivalent chromosomes at metaphase were recorded. It was found that the number of univalent chromosomes ranged from 2 to 12 in 95% of pollen mother cells in diakinesis/metaphase I, and that there was a significant decrease in the number of chiasmata in seeds which were treated with the synthesized nanoparticles as compared to the control. Keywords Asynaptic mutant · Cytotoxicity · Faba bean · Meiosis · Vicia faba · Titanium dioxide · Univalent/bivalent chromosomes Abbreviations AS Asynaptic M Generation of plants NPs Nanoparticles PMCs Pollen mother cells TiO2 Titanium dioxide V. faba Vicia faba
Introduction It is believed by many scientists that nanotechnology can bring the next revolution in biology, physics, medical science and chemistry, etc. (Gottschalk et al. 2013; Sun et al. 2015). In our daily life, titanium dioxide (TiO2) nanoparticles (NPs) are used in paint, glass, cosmetics, solar cells and water-treatment products, and, after the use of these products, TiO2 NPs in large quantities reaches the air, soil * Kalyan Singh Kushwah [email protected] 1
SOS Botany, Jiwaji University, Gwalior, MP 474011, India
and water. As a result, T iO2 NPs come into contact with the environment, plants and living organisms, and enter into the cells that join the cellular elements. Hence, for this reason, nanotoxicity research is drawing attention to plants. T iO2 works as a photocatalyst, and photocatalysts contribute to water treatment by oxidizing organic pollutants in harmless material. TiO2 NPs are favored because of their high photostability and high photon activity, and because they are easily available and affordable. TiO2 compounds can compensate for inadequacies of nitrogen and promote different growth in crops, such as plant biomass and the activity of various enzymes, and increase the chlorophyll content. Titan
Data Loading...
