Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp ( Carassius auratus gibe
- PDF / 589,711 Bytes
- 9 Pages / 547.087 x 737.008 pts Page_size
- 38 Downloads / 174 Views
Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp (Carassius auratus gibelio) Fan Zhang & Kun Hu & Jianzhen Huang & Zhi Tan & Jiming Ruan
Received: 16 December 2019 / Accepted: 7 July 2020 # The Author(s) 2020
Abstract The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABAtransaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD65, GAD67, and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P < 0.05 or P < 0.01). While the expressions of GAD65 and GAD67 in the kidney at 12 h had strikingly upregulated to 13.81 ± 1.06** and 150.67 ± 12.85** times. Treated with the lethal dose of 50% (LD50, 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD65, GAD67, and GABA-T in all tissues were increased significantly (P < 0.01). The results of AVM group showed that the mRNA expressions of GAD65, GAD67, and GABA-T both in the central and peripheral Fan Zhang and Kun Hu are co-first authors. F. Zhang : J. Huang : Z. Tan : J. Ruan (*) Department of Aquaculture, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China e-mail: [email protected] K. Hu National Center for Aquatic Pathogen Collection, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC50, 0.039 mg/L), except for the mRNA inhibitions of GAD65, GAD67, and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 ± 0.02ΔΔ × 10−2, 0.57 ± 0.06ΔΔ × 10−1 and 0.44 ± 0.02ΔΔ × 10−1, respectively (P < 0.01). Keywords Gamma-aminobutyric acid . Difloxacin . Avermectin . Glutamate decarboxylase . GABAtransaminase
Introduction GABA, an important inhibitory neurotransmitter in many organisms, along with glutamate (Glu), is involved in the neuromodulation of most synaptic activity. GABA arises via decarboxylation of L-glutamate by glutamate decarboxylase (GAD) (Chung et al. 1992) and is metabolized subsequently via GABA-transferase (GABA-T) to succinic semialdehyde, which is then oxidized to succinate (Wood et al. 1978). This process would directly affect the accumulations of GABA in organisms. The changes of Glu and GABA in nerve endings would result in rearrangements of the nervous system that increases neural activity (Nasreen et al. 2012). The production and metabolism of GABA can be predicted by observing changes in the expression of enzymes present in nerve endings. However, many factors affect how well GABA works, which include heavy metal (Struzyńska and Sulkowski 2004), antibiotics (Mats
Data Loading...
