Evaluation of in vivo anti-inflammatory and anti-angiogenic attributes of methanolic extract of Launaea spinosa
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Inflammopharmacology
ORIGINAL ARTICLE
Evaluation of in vivo anti‑inflammatory and anti‑angiogenic attributes of methanolic extract of Launaea spinosa Muhammad Asif1,5 · Mahrukh1 · Malik Saadullah1 · Hafiza Sidra Yaseen1 · Mohammad Saleem2 · Hafiz Muhammad Yousaf1 · Ikram Ullah Khan1 · Maria Yaseen3 · Muhammad Usman Shams4 Received: 10 November 2019 / Accepted: 31 January 2020 © Springer Nature Switzerland AG 2020
Abstract Launaea spinosa is used as an anti-inflammatory agent traditionally. This study was conducted to evaluate anti-inflammatory and anti-angiogenic activities of methanol extract of Launaea spinosa. Extraction was performed by maceration and the resultant green coloured extract was labelled as Ls.Me. Solubility analysis showed that Ls.Me was miscible with distilled water, normal saline, ethanol and methanol. Metal analysis following acid digestion method exhibited the presence of copper, magnesium, manganese, iron, zinc and calcium. Phytochemical analysis confirmed the presence of different classes of secondary metabolites in Ls.Me. HPLC analysis showed the presence of quercetin, gallic acid, caffeic acid, benzoic acid and sinapic acid in Ls.Me. Data of in vitro antioxidant assays showed moderate antioxidant potential of Ls.Me which was also confirmed by data of in vivo enzymes (SOD, CAT, and TSP) assays. Antimicrobial assays data showed that Ls. Me was active against S.aureus and S.epidermidis (bacterial) as well as C.albicans and A.niger (fungal) strains. Data of acute physio-pathological studies showed no abnormalities in Albino rats up to the dose of 2000 mg/kg of Ls.Me. Acute and chronic inflammatory models were used to evaluate anti-inflammatory effects of Ls.Me. Data of acute studies showed that Ls.Me has the potential to arrest inflammation produced by different mediators in a dose-dependent manner. 200 mg/ kg of Ls.Me was found to produce significantly (p
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