Evaluation of the Association of rs4680 COMT Polymorphism and Clinical Parameters of the Tumor in Colorectal Cancer (a P
- PDF / 313,362 Bytes
- 4 Pages / 594 x 792 pts Page_size
- 38 Downloads / 147 Views
Bulletin of Experimental Biology and Medicine, Vol. 170, No. 2, December, 2020 ONCOLOGY
Evaluation of the Association of rs4680 COMT Polymorphism and Clinical Parameters of the Tumor in Colorectal Cancer (a Pilot Study) T. I. Ivanova, A. L. Potapov, D. V. Zemskova, V. A. Khorokhorina, P. V. Shegai, S. A. Ivanov, and A. D. Kaprin
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 8, pp. 231-235, August, 2020 Original article submitted May 7, 2020 We analyzed association of rs4680 polymorphism of catechol-O-methyltransferase (COMT) gene with clinical parameters of the tumor in patients with colorectal cancer (n=100). Based on the classification of the tumor according to the TNM system, groups were formed taking into account the size and spreading of the primary tumor (T1+T2 vs Т3+Т4) and the presence of regional (N0 vs N1) and distant metastases (M0 vs M1). An association of the AA genotype with an almost 7-fold increased capacity for invasive tumor growth was found (p0.05) is shown, as well as the coincidence of their frequency with that in the European population (according to NCBI). Analysis of associations of rs4680 COMT polymorphism with CRC risk revealed no significant relationship between them (p>0.05) (Table 1). Particular attention was paid to the study of distributions of polymorphic alleles and rs4680 COMT genotypes between patients with different clinical and morphological parameters of tumor development in CRC. Within each parameter, an increase in the incidence of minor allele and homozygous AA genotypes was observed in groups with greater disease progression. A positive association of minor allele A with tumor cell malignancy (moderate and low differentiated vs highly differentiated) (p=0.058) was revealed at the level of a trend (Table 2). The frequency of homozygous AA genotype was higher in the group with more malignant tumors, but the excess was negligible (Table 2). At the trend level, an excess of allele A frequency was observed in the group of patients with Т3+Т4 compared to Т1+Т2 (p=0.066) (Table 2). The AA genotype is significantly associated (p0.05) (Table 2). COMT is important for the metabolism of catecholamines that affect the intensity of metabolic processes in tissues, contribute to the development
The study included 100 patients diagnosed with CRC treated at the A. Tsyb Medical Radiological Research Center hospital and 130 healthy donors; the patients and donors did not significantly differ by age and sex (p=0.40) (Table 1). Based on the TNM classification criteria of the tumor, the groups were formed taking into account the size and spread of the primary tumor (T1+T2 vs Т3+Т4), the presence of metastases in lymph nodes (N0 vs N1), and the presence of distant metastases (M0 vs M1). This pilot study was approved by the Ethics Committee of the A. Tsyb Medical Radiological Research Center (protocol No. 194, January 18, 2017). DNA was extracted from 300 µl whole blood using the Wizard Genomic DNA Purification Kit (Promega). Informed consent form for particip
Data Loading...
