Exogenous calcium alleviates the impact of cadmium-induced oxidative stress in Lens culinaris medic. Seedlings through m
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J. Crop Sci. Biotech. 2012 (December) 15 (4) : 325 ~ 334 DOI No. 10.1007/s12892-012-0065-3 RESEARCH ARTICLE
Exogenous Calcium Alleviates the Impact of CadmiumInduced Oxidative Stress in Lens culinaris Medic. Seedlings Through Modulation of Antioxidant Enzyme Activities Dibyendu Talukdar* Plant Cell and Stress Biology Laboratory, Department of Botany, R.P.M. College, University of Calcutta, Uttarpara, Hooghly 712258, India
Received: June 28, 2012 / Revised: August 14, 2012 / Accepted: August 24, 2012 Ⓒ Korean Society of Crop Science and Springer 2012
Abstract The effect of calcium (Ca) on lentil (Lens culinaris Medic.) seedlings exposed to cadmium (Cd) stress was studied by investigating plant growth and antioxidant enzyme activities. Plants were grown for 14 days in full-strength Hoagland nutrient media supplemented with Cd concentrations of 0, 10, 20, and 40 µM, and on corresponding medium supplied with 5 mM Ca(NO3)2 prior to Cd addition. Increasing Cd led to accumulation of metal and reduced the fresh weight of the shoots more strongly than that of the roots. Cd concentrations of 20 and 40 µM were selected to study its toxic effect on seedlings. Activities of superoxide dismutase, ascorbate peroxidase, catalase, dehydroascorbate reductase, and glutathione reductase decreased at much higher magnitude in the shoots than those observed in the roots under Cd exposure. Failure of antioxidant defense in scavenging of reactive oxygen species was evidenced by abnormal rise in H2O2, resulting in enhancement of lipid peroxidation and membrane electrolyte leakage as the marks of Cd-induced oxidative stress in lentil seedlings. Ca priming in the media significantly reduced the Cd accumulation and considerably alleviated the adverse impact of Cd treatment by modulating the antioxidant enzyme activity. Mitigation of Cd-induced stress by Ca application was strongly suggested by declining levels of H2O2 and consequent lowering of oxidative damage of membrane. Consequently, this enhanced fresh mass of plant parts as the sign of Ca-mediated normal growth in Cd-treated lentil seedlings. Key words : cadmium, calcium, growth, Lens culinaris, oxidative stress, reactive oxygen species
Introduction Cadmium (Cd) is a non-essential element for plant metabolism even though it is rapidly taken up by plant roots and can be loaded into the xylem for its transport into the leaves (Sanita di Toppi and Gabrielli 1999; Wagner 1993). Cd accumulation is very toxic to plants, causing poor growth and low biomass accumulation through interference in several metabolic processes, such as water and mineral uptake (Sandalio et al. 2001), photosynthesis (Mobin and Khan 2007), enzyme activity (Clemens 2006; Siddiqui et al. 2012), and cellular redox homeostasis (Ortega-Villasante et al. 2005; RomeroDibyendu Talukdar (
) E-mail: [email protected] Phone: 910923099774, 910332663-0191
The Korean Society of Crop Science
Puertas et al. 2004). Moderate Cd contamination of arable soils can result in considerable Cd accumulation in edible parts of cr
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