Expression analysis of sweetpotato NADPH oxidase-encoding Rboh genes in response to infection with the root-knot nematod
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Plant Biotechnology Reports https://doi.org/10.1007/s11816-020-00631-8
SHORT COMMUNICATION
Expression analysis of sweetpotato NADPH oxidase‑encoding Rboh genes in response to infection with the root‑knot nematode Meloidogyne incognita Sang Hyeon Lee1 · Donghwan Shim2 · Kang‑Lok Lee1 · Ki Jung Nam1 · Jung‑Wook Yang3 · Jeung Joo Lee4 · Yun‑Hee Kim1 Received: 31 March 2020 / Accepted: 25 May 2020 © Korean Society for Plant Biotechnology 2020
Abstract Sweetpotato (Ipomoea batatas L.) is a globally important food crop that is susceptible to infestation with the root-knot nematode Meloidogyne incognita, which can cause substantial crop losses. Previous transcriptomic analysis of the roots of susceptible and resistant sweetpotato cultivars identified genes that were likely to contribute to protection against root-knot nematode. The identified genes included respiratory burst oxidase homolog (Rboh) genes, which encode NAPDH oxidase homologs in plants. In this study, the role of Rboh genes in sweetpotato defense responses during root-knot nematode infection was examined in susceptible and resistant cultivars. Comparison with Arabidopsis Rboh genes identified groups of sweetpotato genes orthologous to AtRboh B, E, F, and G. Of these, the group resembling AtrbohB contained the highest number of unigenes, nine, and these genes were highly responsive to nematode infection in sweetpotato roots. These results indicate that Rboh genes play roles in protecting sweetpotato roots against root-knot nematode infection. Keywords Defense signaling · Root-knot nematodes · Sweetpotato · NADPH oxidase · Transcriptome Abbreviations Rboh Respiratory burst oxidase homologs RKN Root-knot nematodes ROS Reactive oxygen species
Sang Hyeon Lee and Donghwan Shim contributed equally to this work. * Yun‑Hee Kim [email protected] 1
Department of Biology Education, IALS, Gyeongsang National University, Jinju 660‑701, Republic of Korea
2
Department of Forest Genetic Resources, National Institute of Forest Science, Suwon 16631, Republic of Korea
3
National Institute of Crop Science, Rural Development Administration, Suwon, Republic of Korea
4
Department of Plant Medicine, IALS, Gyeongsang National University, Jinju, Republic of Korea
Introduction NADPH oxidase is a multigenic enzyme complex that is involved in the production of reactive oxygen species (ROS) in the immune responses of various organisms, including plants (Segal et al. 2012). NADPH oxidases catalyze the transfer of electrons to diatomic oxygen to generate superoxide anion radicals (O2−) (Lambeth 2004). The generation of superoxide initiates a cascade of reactions that results in the generation of several highly reactive oxygen species. Respiratory burst oxidase homologs (Rbohs) are plant homologs of NADPH oxidases. Rbohs generate ROS in response to attack by fungal and bacterial pathogens (Torres et al. 2002), and genetic disruption of specific Rboh genes alters plant responses to pathogens (Lamb and Dixon 1997; Yoshioka et al. 2003; Marino et al. 2012). Arabidopsis c
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