Fungus mediated biogenic synthesis and characterization of chitosan nanoparticles and its combine effect with Trichoderm
- PDF / 1,905,138 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 101 Downloads / 259 Views
RESEARCH ARTICLE
Fungus mediated biogenic synthesis and characterization of chitosan nanoparticles and its combine effect with Trichoderma asperellum against Fusarium oxysporum, Sclerotium rolfsii and Rhizoctonia solani Sarodee Boruah1 · Pranab Dutta2 Received: 15 July 2020 / Revised: 8 October 2020 / Accepted: 10 October 2020 © Indian Phytopathological Society 2020
Abstract In the present study, chitosan nanoparticles were synthesized from four different fungal sources viz., Fusarium oxysporum, Metarhizium anisopliae, Beauveria bassiana and Trichoderma viride and from one commercial source of chitosan.UV–VIS spectroscopy study of synthesized nanoparticles showed absorption peaks at the range of 310.02 to 342.00 nm. Functional groups of chitosan nanoparticle as OH, N–H, C–H, C=O, C–O, C–N and P=O were confirmed by FTIR study. Surface properties of the synthesized nanoparticles were found to be positively charged and were found stable in nature. Electron microscopy study showed that synthesized nanoparticles are nearly spherical in shape. DLS analysis showed the average size of the chitosan nanoparticles synthesized from F. oxysporum, M. anisopliae, B. bassiana, T. viride and commercial products as 273.20, 172.50, 78.36, 89.03 and 300.10 nm respectively. Compatibility study of chitosan nanoparticles with T. asperellum showed highly compatible reaction with chitosan nanoparticles at the tested concentration. In vitro assay against Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii showed combination of T. asperellum and chitosan nanoparticle as superior in suppression of mycelial growth of the pathogens as compared to Trichoderma alone and carbendazim @0.1%. Keywords Chitosan nanoparticle · Fusarium oxysporum · Rhizoctonia solani · Sclerotium rolfsii · Trichoderma asperellum
Introduction Fusarium oxysporum Schlecht, Sclerotium rolfsii Sacc and Rhizoctonia solani Kuehn are the three important soil borne fungal plant pathogens. Among these, Fusarium species is one of the main and best known soil borne plant pathogens in terms of economical damage in agricultural productions all over the world (Saremi et al. 2010; Bentley et al. 2006; Bockus et al. 2007). Fusarium spp. causes significant damage to the crops such as potato, pea, bean, wheat, corn and rice with yield losses upto 30–70% (Saremi et al. 2011) and the wilt caused by F. oxysporum remains as a major threat to the vegetable growers worldwide. Soil borne nature of * Pranab Dutta [email protected] 1
Krishi Vigyan Kendra Tinsukia, Assam Agricultural University, Jorhat, Assam, India
School of Crop Protection, College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University (Imphal), Umroi Road, Umiam 793103, Meghalaya, India
2
the pathogen coupled with its ability to survive as chlamydospores for about 3 decades in infested plant debris or in the roots of alternate hosts makes it highly potential for infecting crop plants (Saikia 2004). Similarly, S. rolfsii (Teleomorph: Athelia rolfsii) and R. solani Kuehn
Data Loading...
