Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant
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ORIGINAL PAPER
Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant A. Lebeau • M. Gouy • M. C. Daunay • E. Wicker • F. Chiroleu • P. Prior • A. Frary J. Dintinger
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Received: 6 April 2012 / Accepted: 16 August 2012 / Published online: 29 August 2012 Ó Springer-Verlag 2012
Abstract Resistance of eggplant against Ralstonia solanacearum phylotype I strains was assessed in a F6 population of recombinant inbred lines (RILs) derived from a intra-specific cross between S. melongena MM738 (susceptible) and AG91-25 (resistant). Resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial colonization index, as assessed in greenhouse experiments conducted in Re´union Island, France. The AG91-25 resistance was highly efficient toward strains CMR134, PSS366 and GMI1000, but
Communicated by I. Paran.
Electronic supplementary material The online version of this article (doi:10.1007/s00122-012-1969-5) contains supplementary material, which is available to authorized users. A. Lebeau M. Gouy E. Wicker (&) F. Chiroleu J. Dintinger (&) CIRAD, UMR Peuplements ve´ge´taux et Bioagresseurs en Milieu Tropical (PVBMT), 7 chemin de l’IRAT, 97410 Saint Pierre, La Re´union, France e-mail: [email protected] J. Dintinger e-mail: [email protected] M. C. Daunay Unite´ de Ge´ne´tique et Ame´lioration des Fruits et Le´gumes, Institut National de la Recherche Agronomique (INRA), Centre d’Avignon, UR1052, Domaine St Maurice, BP94, 84143 Montfavet, France P. Prior INRA/CIRAD, UMR PVBMT, 7 Chemin de l’IRAT, 97410 Saint-Pierre Cedex, La Re´union, France A. Frary Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir 35430, Turkey
only partial toward the highly virulent strain PSS4. The partial resistance found against PSS4 was overcome under high inoculation pressure, with heritability estimates from 0.28 to 0.53, depending on the traits and season. A genetic map was built with 119 AFLP, SSR and SRAP markers positioned on 18 linkage groups (LG), for a total length of 884 cM, and used for quantitative trait loci (QTL) analysis. A major dominant gene, named ERs1, controlled the resistance to strains CMR134, PSS366, and GMI1000. Against strain PSS4, this gene was not detected, but a significant QTL involved in delay of disease progress was detected on another LG. The possible use of the major resistance gene ERs1 in marker-assisted selection and the prospects offered for academic studies of a possible gene for gene system controlling resistance to bacterial wilt in solanaceous plants are discussed.
Introduction The causal agent of bacterial wilt disease (BW), Ralstonia solanacearum, ranks among the most devastating pathogens in important agricultural solanaceous crops such as potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), pepper (Capsicum annuum) and tobacco (Nicotiana spp.) (Food and Agricultural Organization FAO, http://faostat.fao.org/faostat). This bacterium has
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