Human Embryonic Stem Cell Protocols
The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of
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Kursad Turksen Editor
Human Embryonic Stem Cell Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Human Embryonic Stem Cell Protocols Third Edition
Edited by
Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
Editor Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2667-1 ISBN 978-1-4939-2668-8 (eBook) DOI 10.1007/978-1-4939-2668-8 Library of Congress Control Number: 2015947127 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface In spite of ethical and political concerns regarding the use of human embryonic stem cells, their potential to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. In both cases, delineating the steps in and identifying the molecules responsible for the commitment and differentiation of these pluripotent cells to different cell fates remains crucial. With this in mind, I felt that it would be timely to collect some of the most interesting and useful protocols that have emerged during the last several years. I am therefore extremely pleased and thankful to those groups who agreed to share their valuable protocols with others interested in exploring stem cell biology questions in a research setting. Once again, I would like to acknowledge John Walker, Editor in Chief of the series, for continuously fostering my efforts in this series. I am also
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