Identification and expression profile of microRNA in seven tissues of the Golden snub-nosed monkey ( Rhinopithecus roxel
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ORIGINAL ARTICLE
Identification and expression profile of microRNA in seven tissues of the Golden snub‑nosed monkey (Rhinopithecus roxellanae) Qiao Yang1 · Jianqiu Yu2 · Lan Jiang1 · Xuanzhen Liu2 · Fangyuan Liu1 · Yansen Cai1 · Lili Niu2 · Megan Price1 · Jing Li1 Received: 11 April 2020 / Accepted: 26 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract MicroRNAs (miRNAs) are key in the post-transcriptional regulation of gene expression and thus characterization of miRNAs and investigation of the relative abundance and specificity of tissue expression are essential for understanding gene expression in the golden snub-nosed monkey (GSM, Rhinopithecus roxellanae). Here, we report the first dataset of GSM miRNAs where we identified 460 miRNAs in seven tissues, with 246 conserved known mature miRNAs and 214 novel mature miRNAs. We determined miRNA abundance and expression in the seven tissues using a Tissue Specificity Index score and found that most novel GSM miRNAs showed a highly tissue-specific expression pattern. In particular, 67 novel miRNAs and the miR-34 family were expressed in abundance only in the lung. Five known miRNAs were highly abundant in digestive organs such as the pancreas and liver, and four novel miRNAs were highly expressed in the heart and muscle. Annotation of target genes of GSM miRNAs indicated that target genes were enriched in many important pathways, such as the HIF-1 signaling pathway and xenobiotic biodegradation-related pathways. Collectively, these results emphasize that miRNAs play important roles in GSM diet and high-elevation adaptation regulation. In summary, this study provides essential information on GSM miRNAs and will benefit further investigations of the function and mechanism of miRNAs in controlling gene expression in the GSM. Keywords microRNA · Rhinopithecus roxellanae · Tissue specificity · Expression profile
Introduction The golden snub-nosed monkey (Rhinopithecus roxellanae, GSM) is a Chinese endemic primate found exclusively in high elevation montane forests (> 3000 m) in the Minshan Mountains (Sichuan and Gansu Provinces), Qinling Mountains (Shanxi Province) and Shennongjia Mountains (Hubei Province) (Luo et al. 2015; Zhou et al. 2014). The Communicated by Stefan Hohmann. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00438-020-01720-7) contains supplementary material, which is available to authorized users. * Jing Li [email protected] 1
Key Laboratory of Bio‑Resources and Eco‑Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, China
The Chengdu Zoo, Institute of Wild Animals, Chengdu, China
2
monkey was named due to the orange-tan to golden fur on the head and back. The GSM has forestomach fermentation similar to ruminants and is highly adapted to folivory, eating leaves and seeds rather than the fruit and insect diet of many monkeys. The species has been listed as Endangered on the International Union for Conservation
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