Immunogenicity of Shigella sonnei outer membrane vesicles extracted in different environmental conditions
- PDF / 662,945 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 82 Downloads / 171 Views
ORIGINAL ARTICLE
Immunogenicity of Shigella sonnei outer membrane vesicles extracted in different environmental conditions Samaneh Sarvary 1 & Seyed Latif Mousavi Gargari 1 & Shahram Nazarian 2 & Razieh Rezaei Adriani 1 & Nafiseh Noroozi 1 Received: 21 December 2019 / Accepted: 16 September 2020 # Institute of Molecular Biology, Slovak Academy of Sciences 2020
Abstract Shigellosis, acute inflammatory bacillary dysentery diseases, is an important agent in the mortality of children in developing countries. Annually 165 million people are being infected leading to 1.2 million deaths around the world. Although vaccination appears to be the only rational prophylactic approach to control shigellosis, currently, there is no safe and efficacious vaccine available in the market. Here in this study, we investigated the protection conferred by a new vaccine based on nanoparticles containing outer membrane vesicles (OMVs) of Shigella sonnei in an experimental model of shigellosis in mice. OMVs extracted by deoxycholate detergent were encapsulated in chitosan-TPP nanoparticles prepared by an ion gelation method and coated with an enteric polymer (NP-OMVs). NP-OMVs were spherical and homogeneous with a mean size of 344.7 nm (PdI = 0.2). BALB/c mice (females, 9-week-old, 25 ± 1 g) were immunized intraperitoneally (i.p.) with 20 µg of OMVs or by intragastric route (i.g.) with 50 µg of free or encapsulated OMVs. The protein concentrations of OMVs were 0.65 mg/mL and 1.02 mg/mL for bacteria grown at 37 C and 42 C respectively. OMVs loaded into nanoparticles were homogeneous and spherical, with a size of 344 nm. The encapsulation efficiency of NP was 85%. Antibody titers in immunized mice sera after 78 days revealed a persistent immune response. Free OMVs were able to protect against infection when it was administered by the intraperitoneal route. Oral administration of NP-OMVs showed better protection whereas free OMVs did not protect against infection. The results obtained in this research place OMVs among promising candidates to be used for vaccination. Keywords Shigella sonnei . Shigellosis . Chitosan . Vaccine candidate . OMVs
Abbreviations OMVs Outer membrane vesicles NP-OMVs Nanoparticle-outer membrane vesicles i.p. Intraperitoneal i.g. Intragastric CS Chitosan LPS Lipopolysaccharide LB Luria-Bertani DLS Dynamic light scattering RT Room temperature * Seyed Latif Mousavi Gargari [email protected] * Shahram Nazarian [email protected] 1
Department of Biology, Shahed University, Tehran-Qom Expressway, 3319118651 Tehran, Iran
2
Department of Biology, Faculty of Science, Imam Hussein University, Tehran, Iran
IpaD nOMVs sOMVs TEM WHO Eu
Invasion plasmid antigen D Normal OMVs Stress OMVs Transmission Electron Microscopy World Health Organization Eudragit
Introduction Diarrhea disease threats the lives of at least five million children per year in developing countries and shigellosis is responsible for approximately 165 million cases, of which 1.2 million are fatal (Puzari et al. 2018; Wang et al. 2016). Shigella flexner
Data Loading...
