Immunomodulatory effect of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in allergic conditions in vitro and in vivo
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ORIGINAL RESEARCH
Immunomodulatory effect of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in allergic conditions in vitro and in vivo Akira Iwamoto . Yuichi Inoue . Hirofumi Tachibana . Hiroharu Kawahara
Received: 10 March 2020 / Accepted: 4 November 2020 Ó Springer Nature B.V. 2020
Abstract We found that strawberry extract suppressed immunoglobulin (Ig) E production in vitro and in vivo, and identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as one of the IgE suppressor in the extract. We report here the effect of GAPDH on various Ig productions in vitro and in vivo. GAPDH suppressed IgE and enhanced IgA, IgG and IgM productions in ovalbumin (OVA)-stimulated human peripheral blood mononuclear cells. Oral administration of GAPDH at 10 mg/kg/day to OVA-induced allergy model mice tended to decrease total IgE level and increase total IgA and IgG levels in sera, and also decreased OVA-specific IgE and IgG levels. It is known that the increase of total IgA as well as the decrease of total and specific IgE is important for alleviating allergic symptoms. In addition, GAPDH accelerated IgA production and increased some cytokine secretions such as IL-4, TGF-b1 and IFN-c in the OVAimmunized mice spleen lymphocytes. These cytokines involved in the class-switching, IgA enhancement, and IgE suppression, respectively, supporting above results.
A. Iwamoto H. Tachibana Division of Applied Biological Chemistry, Department of Bioscience and Biochemistry, Faculty of Agriculture, Kyushu University, 744 Motooka Nishi-ku, Fukuoka 819-0395, Japan Y. Inoue (&) H. Kawahara Department of Creative Engineering, National Institute of Technology, Kitakyushu College, 5-20-1 Shii, Kokuraminami-ku, Kitakyushu, Fukuoka 802-0985, Japan e-mail: [email protected]
Our study suggests a possibility that oral administration of GAPDH may induce the immunomodulation in allergic responses. Keywords GAPDH IgE Immunomodulatory effect Immunoglobulin IgA Abbreviations EGCG (-)-Epigallocatechin-3-O-gallete DHA Docosahexaenoic acid ELISA Enzyme-linked immunosorbent assay EPA Eicosapentaenoic acid FBS Fetal bovine serum GAPDH Glyceraldehyde-3-phosphate dehydrogenase HRP Horseradish peroxidase IFN Interferon Ig Immunoglobulin IL Interleukin i.p. Intraperitoneal IVI In vitro immunization MDP Muramyl dipeptide NAD Nicotinamide adenine dinucleotide PBMC Peripheral blood mononuclear cell PBS Phosphate buffered saline SD Standard deviation SPF Specific pathogen free Th Helper T TGF Tumor growth factor
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Cytotechnology
Introduction Functional foods can improve or keep the physical condition and be also useful for prevention of the symptom onset of disease (Diplock et al. 1999; Roberfroid 1999). They include one or more active constituents that scientifically demonstrated beneficial effects to some symptoms. In recent years, it is reported that a lot of food factors such as (-)epigallocatechin-3-O-gallete (EGCG), pectin, hesperidin, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have
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