Influenza Virus Methods and Protocols
This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, vir
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Yohei Yamauchi Editor
Influenza Virus Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Influenza Virus Methods and Protocols
Edited by
Yohei Yamauchi School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK
Editor Yohei Yamauchi School of Cellular and Molecular Medicine University of Bristol Bristol, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8677-4 ISBN 978-1-4939-8678-1 (eBook) https://doi.org/10.1007/978-1-4939-8678-1 Library of Congress Control Number: 2018949617 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface In 1918 the Spanish Flu pandemic killed an estimated 40 million people around the world. One hundred years later, in 2018, influenza virus still maintains a significant presence in the medical and veterinary spheres. Since the first isolation of a human influenza virus strain in 1933, influenza has been researched intensively and remarkable progress has been made: the recent discovery of bat influenza, decoding of the heterotrimeric influenza A polymerase structure, and identification of the host gene responsible for avian virus polymerase adaptation in mammals, are a few examples. The development of influenza virus reverse genetics in the late 1990s furthered our understanding of viral pathogenesis and viral protein functions. It also promoted the generation of novel vaccine strategies. I
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