Investigating the frequency of free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city,
- PDF / 2,468,380 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 40 Downloads / 149 Views
BMC Research Notes Open Access
RESEARCH NOTE
Investigating the frequency of free‑living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019–2020 Homa Attariani1, Habibollah Turki1, Saeed Shoja2, Abdoreza Salahi‑Moghaddam1, Amin Ghanbarnejad3 and Jebreil Shamseddin1*
Abstract Objective: These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is nec‑ essary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results: Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enabled us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study. Keywords: Free-living amoebae, Acanthamoeba, Water resources Introduction Pathogenic and opportunistic free-living amoebae such as Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri are aerobic, mitochondriate, eukaryotic organisms that occur worldwide and can potentially cause infections in humans and other animals. They are ubiquitous in soil and water resources, aquatic environments, ponds, hot springs, swimming pools, domestic sewage, air, air conditioning chambers, sediments, stagnant water, and artificial humanmade creatures [1]. These amoebae use bacteria, yeasts, and other organisms as food. Unlike real parasites, pathogenic free-living amoebae can complete their life cycle in the environment *Correspondence: [email protected] 1 Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Full list of author information is available at the end of the article
without entering the human and animal host. An increasing number of people with immunodeficiency diseases, including AIDS, treating the patients with corticosteroids or people undergoing chemotherapy, are at increased risk of developing amoebic infections [2, 3]. In Turkey, environmental samples (100%), in USA 2454 tap water samples (51%), water supply in Osaka, Japan (19%), and in 40 water and sanitation facilities samples in Tunisia Hospital (47.6%) Acanthamoeba was found [4–6]. In Iran, some researches covered large areas of Iran to detect various free-living amoeba [7], and some others identified other parasites accompanied by amoeba [8, 9]. Acanthamoeba detection is usually carried out based on the structural characteristics of the cyst through direct microscopic diagnostic methods and culture techniques. This technique has its limitations due to the impacts of cultivation conditions. In recent years, the molecular method has largely solved the pr
Data Loading...
