Magnetic bacterial cellulose nanofibers for nucleoside recognition
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ORIGINAL RESEARCH
Magnetic bacterial cellulose nanofibers for nucleoside recognition Yes¸ eren Saylan . llgım Go¨ktu¨rk . Kristyna Pospiskova . Ivo Safarik . Adil Denizli
Received: 1 May 2020 / Accepted: 29 August 2020 Ó Springer Nature B.V. 2020
Abstract Applications of nanomaterials to biological systems have received increased interest in the past decades. In particular, bacterial cellulose nanofibers can be utilized in a broad range of applications. Biomaterials have been produced on large-scale with high reproducibility by an inspiring method such as molecular imprinting. Herein, for the first time, magnetic bacterial cellulose nanofibers are designed by the molecular imprinting method as a novel adsorbent for selective and efficient recognition of thymidine nucleoside. In this process, magnetic bacterial cellulose nanofibers are silanized with 3-(trimethoxysilyl) propyl methacrylate and further polymerized with a hydrophilic monomer for templating thymidine via metal chelate coordination. They are characterized by several methods and then
applied for thymidine recognition to optimize the adsorption conditions. Hereby, the effecting factors are evaluated, and the highest adsorption capacity is obtained as 431.3 mg/g in pH 9.0 at 25 °C. The selectivity is assessed with competitor nucleosides and highly selective (4.13 and 3.80 times) adsorption of thymidine at the same concentration of other nucleosides (cytidine and uridine) is observed. After multiple adsorption–desorption experiments, the magnetic bacterial cellulose nanofibers also provided high reusability capacity. The present new work holds excellent potential for nucleoside recognition by integrating molecular imprinting with magnetic bacterial cellulose nanofibers.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10570-020-03425-x) contains supplementary material, which is available to authorized users. Y. Saylan l. Go¨ktu¨rk A. Denizli (&) Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey e-mail: [email protected] K. Pospiskova I. Safarik Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic I. Safarik Department of Nanobiotechnology, Biology Centre, ISB, CAS, Ceske Budejovice, Czech Republic
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Cellulose
Graphic abstract
Keywords Magnetic bacterial cellulose nanofiber Molecular imprinting Nucleoside Thymidine
Introduction A five-carbon sugar is combined with the organic base to form a nucleoside by glycoside bond. In nature, highly selective pathways are developed to link nucleosides, specifically with other similar structure nucleosides (Rose and Coe 2008). They act as metabolic precursors in energy metabolism and nucleic acid synthesis and also play a crucial part in the eukaryotes physiology (Boswell-Casteel et al. 2018; Young et al. 2013; Greenhalf et al. 2014). Specific recognition of nucleosides is essential, especially in cancer biomarkers including anti-neop
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