Mesta yellow vein mosaic virus : application of loop-mediated isothermal amplification method to study efficiency of acq
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ORIGINAL RESEARCH ARTICLE
Mesta yellow vein mosaic virus: application of loop-mediated isothermal amplification method to study efficiency of acquisition, retention and transmission by Bemisia tabaci (Hemiptera: Aleyrodidae) in Kenaf P. N. Meena 1,2
&
B. S. Gotyal 2 & S. Satpathy 2
Received: 9 April 2020 / Accepted: 6 October 2020 # African Association of Insect Scientists 2020
Abstract Whitefly is an important pest and vector of many plant begomoviruses. Investigations were carried out to understand the acquisition, retention, transmission, and gender basis transmission efficiency of Mesta yellow vein mosaic virus (MeYVMV) by whitefly. The minimum acquisition access feeding period (AAFP) was 0.10 h which resulted in 11.1% infected plants. Based on LAMP assay and yellowing symptoms, 100% virus acquisition was achieved by whitefly with an exposure of 18 to 48 h. Virus was transmitted in tested plants up to 88.8% after 1 to 10 days retention time of viruliferous whiteflies, whereas no symptoms were observed after 35 days. Fifteen minutes of exposure to viruliferous whiteflies on healthy plants resulted in 11.1% transmission. Cent percent transmission of virus was achieved in plants with 12 h exposure period. Highest virus transmission efficiency of 55.60% was observed in female whitefly compared to 33.3% in single male whitefly. Present study shall help to understand the interaction of insect-plant–virus relationship, epidemiological knowledge, and formulation of management strategies against virus and its vector. Keywords Mesta yellow vein mosaic virus . Bemisia tabaci . Hibiscus cannabinus . LAMP method
Introduction Kenaf (Hibiscus cannabinus L.) an annual, herbaceous, lignocellulosic crop belongs to Malvaceae is grown in several countries including India (Mahadevan et al., 2009). It is mainly grown for bast fibers which provide traditional packaging material and a good quality pulp for production of paper. It is also used as leafy vegetables and seeds are rich in oil with a low level of unsaturated fatty acids with potential medicinal values (Duke 1986; Pal and Jain 1998; Wong et al. 2014). Whitefly (B. tabaci (Gennadius) is a pest of >600 host plants including fiber crops, ornamental plants, vegetables, * P. N. Meena [email protected] 1
ICAR- National Research Centre for Integrated Pest Management, LBS Building, Indian, Agricultural Research Institute, New Delhi 110012, India
2
Crop Protection Division, ICAR-Central Research Institute for Jute and Allied Fibers, Barrackpore, Kolkata, West Bengal 700120, India
weeds and spices (Secker et al. 1998; Martin et al. 2000; Oliveira et al. 2001; Jones 2003; Liu 2007; Zhang et al. 2007; Naranjo and Ellsworth 2009; Wan et al. 2009). B. tabaci is a highly adaptive insect that feeds on various plants (Greathead 1986; Oliveira et al. 2001) and continues to infest new host species (Simmons et al. 2008) vectoring >100 plant viruses (Jones 2003). New biotype of viruses exhibit difference in virus transmission, endosymbionts, developmental rate, transmission efficienc
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