MicroRNAs-1614-3p gene seed region polymorphisms and association analysis with chicken production traits
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ANIMAL GENETICS • SHORT COMMUNICATION
MicroRNAs-1614-3p gene seed region polymorphisms and association analysis with chicken production traits Hong Li & Gui-Rong Sun & Ya-Dong Tian & Rui-Li Han & Guo-Xi Li & Xiang-Tao Kang
Received: 8 November 2012 / Revised: 2 February 2013 / Accepted: 11 February 2013 / Published online: 2 March 2013 # Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2013
Abstract In the present study, a total of 860 chickens from a Gushi–Anka F2 resource population were used to evaluate the genetic effect of the gga-miR-1614-3p gene. A novel, silent, single nucleotide polymorphism (SNP, +5 C>T) was detected in the gga-miR-1614-3p gene seed region through AvaII polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR products sequencing methods. Associations between the SNP and chicken growth, meat quality and carcass traits were performed by association analysis. The results showed that the SNP was significantly associated with breast muscle shear force and leg muscle water loss rate, wing weight, liver weight and heart weight (pA)
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seed region was significantly associated with body weight at 4, 6, 8, 10 and 12 weeks of age and some body size traits (Li et al. 2012). An SNP rs15172520 (http://www.integratomicstime.com/miRNA-SNiPer) within the mature gga-miR-16143p (+5 G>A) sequence (Griffiths-Jones et al. 2008) seed region was found. gga-miR-1614 is located in the intergenic region on chromosome 20, and this SNP has not been studied in detail thus far. We hypothesised that miR-1614-3p may play an important role in the formation of economic phenotypes of chicken breeds. The purpose of this study was to identify the polymorphic G>A SNP and analyse the relationship between the SNP and some performance traits of an F2 resource population. The F2 population of 860 chickens was created from a cross between Gushi chickens, which represent a slowgrowing Chinese native chicken breed, and Anka chickens, which represent a fast-growing broiler, as previously described (Han et al. 2010; Lv et al. 2012). All the chickens were managed under the same environment, with free access to feed and water, and were slaughtered at the age of 84 days. A total of 860 serum blood samples were collected from the F2 individuals and stored at −80 °C. Traits such as liver weight, heart weight, wing weight, weight of the abdominal fat, breast muscle shear force, leg muscle water loss rate and so on were measured after slaughtering. Growth traits including body weight and traits of body size were recorded; body weight was weighed individually every 2 weeks from hatching to slaughter, whereas traits of body size were measured at 4, 8 and 12 weeks (Han et al. 2011, 2012). The genomic DNA from blood was extracted by the phenol–chloroform method (Sambrook and Russell 2001). One hundred DNA samples from the F2 individuals with the same working concentration and of equal amounts were selected randomly to construct a DNA pool, which was Fig. 1 Single nucleotide polymorphism (SNP) locat
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