Modulatory Effect of Rubus fruticosus L. on Liver Biochemical Metabolism and Inflammatory Cytokines in the Diabetic Rats
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RESEARCH PAPER
Modulatory Effect of Rubus fruticosus L. on Liver Biochemical Metabolism and Inflammatory Cytokines in the Diabetic Rats Mahya Soheilifar1 • Abdolhossein Shiravi1 • Naser Mirazi2
•
Vida Hojati1 • Roghayeh Abbasalipourkabir3
Received: 11 March 2020 / Accepted: 29 September 2020 Ó Shiraz University 2020
Abstract Diabetes mellitus is associated with increased oxidative stresses, kidney damage, ocular neuropathy, and atherosclerosis. In the present study, the protective effect of Rubus fruticosus hydroethanolic extract on the biochemical parameters in streptozotocin (STZ)-induced diabetic rats were investigated. In this experimental study, 40 male Wistar rats were divided into five groups (n = 8) as control, diabetic (STZ-injection 55 mg kg-1 of body weight, intraperitoneally (i.p.)), and treatment 1, 2, and 3 which treated by R. fruticosus extract (50, 100 and 200 mg kg-1, i.p.). After 4 weeks, the animals were deeply anesthetized and their blood samples were collected from the heart directly for the measurement of biochemical parameters and inflammatory cytokines. The findings indicate that the serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), ceruloplasmin (Cp), copper (Cu), tumor necrosis factor (TNF)-a, and interleukin (IL)-6 increased significantly after the onset of diabetes (P \ 0.001 for all assayed parameters). Treatment by R. fruticosus extract at high dose (200 mg kg-1) reduced the serum levels of ALT, AST, ALP, GGT, Cp, Cu, TNF-a, and IL-6 (P \ 0.001, for all parameters) significantly compared to the diabetic group. Overall, the administration of the R. fruticosus extract can ameliorate some of the adverse effects of STZinduced diabetes among the male rats that received this treatment. Keywords Diabetes mellitus Rubus fruticosus Liver enzyme Inflammatory markers Streptozotocin
1 Introduction Diabetes mellitus (DM) is a disorder of glucose metabolism by reducing insulin production and its uptakes by tissues, which is posed as a major global health problem (Gomar et al. 2014). Deficiency in the trace elements metabolism like copper (Cu) and iron has been reported in DM (Qiu et al. 2017). Cu’s binding to the glycosylated proteins result in reactive oxidation and active resuscitation, resulting in greater exacerbation of & Naser Mirazi [email protected] 1
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2
Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
3
Department of Clinical Biochemistry, School of Medicine, Hamedan University of Medical Sciences and Health Services, Hamadan, Iran
oxidative stress and the production of the free radicals in the DM (Qiu et al. 2017). Streptozotocin (STZ)-induced diabetes causes malfunctions in Cu metabolism and defense against oxidants (Karganov et al. 2020). The imbalance in the Cu leads to the increased oxidative damage in the tissue an
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