Molecular analysis of ERF subfamily genes during coffee somatic embryogenesis
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PLANT TISSUE CULTURE
Molecular analysis of ERF subfamily genes during coffee somatic embryogenesis Matheus Martins Daude 1 & Natália Chagas Freitas 2 & Solange Aparecida Ságio 3 & Luciano Vilela Paiva 2 & Horllys Gomes Barreto 1 Received: 21 May 2020 / Accepted: 25 August 2020 / Editor: Todd Jones # The Society for In Vitro Biology 2020
Abstract With ample cultivation and economic potential, coffee is one of the main commodities in the world. Despite its vast cultivation, there remain problems related to its productivity as a consequence of pests and diseases, questions regarding its physiology, and difficulty with its propagation. Due to such challenges, the application of biotechnological tools, such as tissue culture and molecular markers, has become essential in the search for improvements to coffee cultivation. Among tissue culture techniques, somatic embryogenesis is considered the best form of micropropagation in vitro since it is possible to produce plants that are identical to the parent plant. It is therefore essential to identify the genes involved in the process of somatic embryogenesis, as well as to understand their functions. Different studies have shown the expression of ethylene response factor (ERF) genes in different tissues and under different conditions, mainly in response to biotic and abiotic stresses. However, little is known about their role during somatic embryogenesis. Thus, this study is aimed to identify and analyze the expression patterns of ERF subfamily genes during Coffea arabica L. somatic embryogenesis. The EST-contig6, 9, and 27 were confirmed to be the ethylene response factor 8, 13, and 12 genes from Coffea arabica L., respectively, and their relative expression analysis suggests that they may be involved in coffee somatic embryogenesis. The expression patterns of ethylene response factor 8 and 13 in embryogenic calluses and embryogenic cell suspensions showed that these genes can potentially act as somatic embryogenic markers. Keywords Coffea arabica L. . Embryogenic potential . RT-qPCR . Transcriptional analysis . Molecular marker
Introduction Coffee has become one of the main commodities in the world due to its economic potential and broad cultivation (MAPA: Ministério da Agricultura, Pecuária e Abastecimento, 2017). Despite extensive cultivation and research on the species, due to its economic relevance, Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11627-020-10120-w) contains supplementary material, which is available to authorized users. * Horllys Gomes Barreto [email protected] 1
Laboratory of Molecular Analysis, Department of Life Science, Federal University of Tocantins, Palmas, TO, Brazil
2
Central Laboratory of Molecular Biology, Department of Chemistry, Federal University of Lavras, Lavras, MG, Brazil
3
Master in Agroenergy, Federal University of Tocantins, Palmas, TO, Brazil
there are still problems related to its productivity as a consequence of pests and diseases (Carvalho et al. 2012; Carvalho et al
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