Molecular testing of Klebsiella pneumoniae contaminating tissue allografts recovered from deceased donors
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Molecular testing of Klebsiella pneumoniae contaminating tissue allografts recovered from deceased donors Zohreh Ghalavand . Alireza Heidary Rouchi . Hassan Bahraminasab . Elham Ravanasa . Elnaz Sadat Mirsamadi . Narges Nodeh Farahani . Bahram Nikmanesh
Received: 13 August 2017 / Accepted: 15 January 2018 Ó Springer Science+Business Media B.V., part of Springer Nature 2018
Abstract Microbiological screening of tissue allografts is crucial to prevent the transmission of bacterial and fungal infections to transplant recipients. Klebsiella was the most prevalent and resistant contaminating microorganism observed in our setting in the Iranian Tissue Bank. This study was conducted to determine the presence of extended-spectrum blactamase (ESBL) genes, antimicrobial resistance patterns of Klebsiella pneumoniae isolates, and their clonal relationships in allograft materials. K. pneumoniae contaminating bone and other tissue allografts recovered from deceased donors were identified and ESBL isolates were detected using a phenotypic confirmatory method. Antimicrobial susceptibility testing was carried out using the disk diffusion method. Distribution of ESBL genes and molecular
typing were performed using polymerase chain reaction (PCR) and Repetitive-element (rep-PCR) methods. Of 3828 donated tissues, 51 (1.3%) were found contaminated by K. pneumoniae isolates. Compared to tissue allografts from brain-dead, heart-beating tissue donors, allografts from donors with circulatory cessation were associated with a higher risk of K. pneumoniae contamination [odds ratio (OR), 1.2 (CI 95% 0.9–2.3) (P value \ 0.001)]. Half of the isolates produced ESBL, and the rate of susceptibility to cephalosporins was 51%. Among isolates, 22 (43.1%) harbored CTX-M, 31 (60.8%) SHV, and 9 (17.6%) harbored TEM types. The rep-dendrogram indicated that clones having identical or related strains with a similar antibiotype were isolated in the same period. This study provides evidence that a single clone of K.
Z. Ghalavand E. S. Mirsamadi Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran e-mail: [email protected]
N. Nodeh Farahani Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran e-mail: [email protected]
E. S. Mirsamadi e-mail: [email protected] A. Heidary Rouchi H. Bahraminasab E. Ravanasa Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran e-mail: [email protected]
B. Nikmanesh (&) Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, No. 17, Ghods Ave, Enghelab St., Tehran 1419733151, Iran e-mail: [email protected]
H. Bahraminasab e-mail: [email protected] E. Ravanasa e-mail: [email protected]
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Cell Tissue Bank
pneumoniae contaminated tissue allografts recovered from many different donors. A single clone found on tissues from several donors suggests contamination of tissues from a sing
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