Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filamen
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FISH PARASITOLOGY - ORIGINAL PAPER
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil Diego Henrique Mirandola Dias Vieira 1 & Luís Filipe Rangel 2 & Vinicius Panciera Tagliavini 1 & Vanessa Doro Abdallah 3 & Maria João Santos 2 & Rodney Kozlowiski de Azevedo 3 Received: 2 December 2019 / Accepted: 5 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The present study describes Henneguya tietensis n. sp. parasitizing Prochilodus lineatus (Valenciennes, 1837) from Brazil, through morphological and molecular analysis. Myxospores were found in the gill tissue of P. lineatus with a prevalence of 10%. The myxospores consisted of two elongate, elliptical shell valves each with a long, tapering caudal appendage. The morphology was consistent with Henneguya and the myxospore measured as follows (mean ± SD): total length 55.5 ± 2.1 μm, body length 16.2 ± 1.1 μm, body width 5.5 ± 0.1 μm, caudal appendages length 39.0 ± 2.0 μm. The polar capsules were 7.3 ± 0.2 μm long, 1.7 ± 0.2 μm wide and contained a polar filament coiled 11 to 13 turns. Histological analysis showed the plasmodia developing in the middle region of each gill filament and caused a focally extensive distension by forming a spaceoccupying mass within the gill filament. The ultrastructural analysis allowed the observation of the presence of mature myxospores throughout the plasmodium. Phylogenetic analysis with the SSU rDNA gene showed Henneguya tietensis n. sp. as a sister species of the subclade formed by Henneguya piaractus and Henneguya brachypomus that infect fishes of the genus Piaractus. The genetically closest species was H. piaractus, which showed a similarity of 82.4%. Using molecular and morphological characterization, the myxozoan parasite described herein represents a new species of the genus Henneguya. Keywords Characiformes . Henneguya . Myxobolidae . Phylogeny . Tietê River
Introduction Myxozoans are cnidarians with a complex life cycle, alternating between vertebrate hosts in the myxospore phase and invertebrate hosts in the actinospore (Okamura et al., 2015). Section Editor: Sascha L Hallett * Diego Henrique Mirandola Dias Vieira [email protected] 1
Departament of Parasitology, Universidade Estadual Paulista “Júlio de Mesquita Filho” – Unesp, R. Prof. Dr. Antônio Celso Wagner Zanin, 250 - Distrito de Rubião Junior, Botucatu, São Paulo CEP: 18618-689, Brazil
2
Faculdade de Ciências e CIIMAR, Universidade do Porto, Porto, Portugal
3
Docente do Programa de Pós-graduação em Análise de Sistemas Ambientais, Centro Universitário CESMAC, Maceió, Alagoas, Brazil
Several species of myxozoans have been reported as important fish pathogens (Molnár et al. 1998; El-Mansy and Bashtar 2002; Yokoyama et al. 2003; Griffin et al. 2008). Within the genus Henneguya Thélohan, 1892, several species are pathogenic to their hosts (Eiras and Adriano 2012). Henneguya pellis Minchew 1977, which ca
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