Oral Biology Molecular Techniques and Applications
This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and
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Gregory J. Seymour Mary P. Cullinan Nicholas C.K. Heng Editors
Oral Biology Molecular Techniques and Applications Second Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Oral Biology Molecular Techniques and Applications Second Edition
Edited by
Gregory J. Seymour Faculty of Dentistry, University of Otago, Dunedin, New Zealand
Mary P. Cullinan Department of Oral Sciences, Faculty of Dentistry, University of Otago, Dunedin, New Zealand
Nicholas C.K. Heng Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand
Editors Gregory J. Seymour Faculty of Dentistry University of Otago Dunedin, New Zealand
Mary P. Cullinan Department of Oral Sciences, Faculty of Dentistry University of Otago Dunedin, New Zealand
Nicholas C.K. Heng Faculty of Dentistry, Sir John Walsh Research Institute University of Otago Dunedin, New Zealand
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6683-7 ISBN 978-1-4939-6685-1 (eBook) DOI 10.1007/978-1-4939-6685-1 Library of Congress Control Number: 9781493967384 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Example of a bead experiment combined with in situ hybridization (ISH) analysis to study gene expression in embryonic tissue explants. The image shows the effects of BMP2 beads on ld1 gene expression in explants of calvarial mesenchyme. Photograph provided by D. Rice and K. Närhi. The bead and ISH experiments are described in Chapter 20. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface It is widely accepted that “evidence-based
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