Plasma-activated water regulates root hairs and cotyledon size dependent on cell elongation in Nicotiana tabacum L.
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Plant Biotechnology Reports https://doi.org/10.1007/s11816-020-00641-6
ORIGINAL ARTICLE
Plasma‑activated water regulates root hairs and cotyledon size dependent on cell elongation in Nicotiana tabacum L. Young Koung Lee1 · Junghyun Lim1 · Eun Jeong Hong1 · Seong Bong Kim1 Received: 25 June 2020 / Accepted: 1 September 2020 © Korean Society for Plant Biotechnology 2020
Abstract Tobacco is an excellent model plant to study developmental processes and cellular biology. Surface dielectric barrier discharge (sDBD) was used to generate plasma activated water (PAW), and the PAW was indirectly applied to study tobacco plant growth during the early developmental stages. Solid media were treated with the PAW for 8- and 18-day-old seedlings, and the effects on both the vegetative and root phenotypes were observed during the early developmental stages. In the vegetative tissue, the cotyledon size was large for the seedling that was cultivated by plasma activated water for the 12 min PAW (PAW12) treatment. The PAW dramatically elongated the cotyledon by 40% due to increased cell expansion in the leaflength direction, not cell proliferation. In addition to the vegetative tissue, the root length due to the PAW was significantly enhanced at the primary root with elongated root hairs. According to molecular analysis, the NT_ COBRA-LIKE 9 (COBL) and two NT_ XYLOGLUCAN ENDO-TRANSGLYCOSYLASE/HYDROLASE (XTH) genes, NT_XTH9 and NT_XTH15, were significantly over-expressed by the PAW treatment compared with untreated deionized water (DW), indicating that the PAW effects are from modulating the expression of target genes. These results demonstrate that PAW might promote tobacco plant growth both in the cotyledon and root hair tissues via the NT_COBL, NT_XTH9 and NT_XTH15 genes. Keywords Surface dielectric barrier · Plasma-activated water · Tobacco · Cell elongation · XYLOGLUCAN ENDOTRANSGLYCOSYLASE/HYDROLASE (XTH)
Introduction Tobacco (Nicotiana tabacum L.) has been one of the wellstudied annual dicot plants. It is commonly cultivated for its economic value commercially grown worldwide to produce tobacco leaf. Besides economic reasons, tobacco is quite an attractive crop to research as a model plant. A model plant in the field of plant science is Arabidopsis thaliana which has a small genome size (~ 135 Mb), a small plant size enabling its growth in a growth chamber, and a lot of seed production for genetic studies. Arabidopsis has a stable and easy floral dipping transformation method for gene function studies Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11816-020-00641-6) contains supplementary material, which is available to authorized users. * Young Koung Lee [email protected] 1
Plasma Technology Research Center, National Fusion Research Institute, 37, Dongjangsan‑ro, Gunsan‑si, Jeollabuk‑do 54004, Republic of Korea
(Zhang et al. 2006; Clough and Bent 1998). However, it is not a crop and has limitations for direct application to agriculture. Tobacco can be simply transfor
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