Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source
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ORIGINAL PAPER
Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source Vijay Rayasam1 · Pooja Chavan2 · Tarika Kumar3 Received: 27 January 2020 / Revised: 11 July 2020 / Accepted: 22 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Many bacteria produce polyhydroxyalkanoates (PHAs) when exposed to stressful conditions. It is a known fact that PHAs have the potential to replace petrochemical-based plastics as they are biodegradable, biocompatible, and thermoprocessible materials. The study focusses on producing PHA from microbes isolated from polluted environments and pomegranate peels were utilized as a unique carbon source. This was done to ensure reduction in the cost of the substrate that has not yet been reported as a substrate for PHA production. A comparative study was also done with Cupriavidus necator, the reference strain. Out of many bacterial strains, isolated, eight of these were found to have ability to produce PHA. Pomegranate peel was substituted as carbon source in the medium and all bacterial isolates along with reference strain were used to test their ability to produce PHA from waste. Cupriavidus necator, the reference strain, yielded 71% PHA. Bacillus halotolerans DSM8802 yielded 83% at 1:1:: C:N ratio at 72 h. Keywords Bioplastics · Biopolymers · Biodegradable · Microbial polysaccharides · Polyhydroxyalkanoates · Zero wastage
Introduction Bio-based plastics have received tremendous attention in recent years because of their high molecular weight, biodegradability, non-toxicity, thermoplasticity, biocompatibility, elastomeric properties and because of their production from Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01995-9) contains supplementary material, which is available to authorized users. * Tarika Kumar [email protected] Vijay Rayasam [email protected] Pooja Chavan [email protected] 1
Department of Biotechnology, REVA University, Rukmini Knowledge Park, Kattigenahalli, Bangalore 560064, India
2
Parul Institute of Applied Sciences (PIAS), Parul University, Post Limda, Waghodia, Vadodara, Gujarat 391760, India
3
Department of Environmental Studies, Faculty of Science, Sir CV Raman Building, The Maharaja Sayajirao University, Sayajigunj, Vadodara, Gujarat 390002, India
renewable carbon sources. The research in the packaging industry develops and also promotes the use of bioplastics as they prove helpful in reducing waste disposal and are better substitutes for petroleum which is a non-renewable resource. The issue of a large quantity of waste generated due to the non-degradable food packaging materials has ignited the need for research on biopolymers (Ruban 2009; Souza et al. 2010; Azeredo et al. 2012; Gabor et al. 2012). The petrochemical-based plastic materials are the most difficult to manage waste on planet earth because the fact that they are non-biodegradable but ha
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