Polymeric colloidal nanocarriers entrapped with Centella asiatica extract
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Polymeric colloidal nanocarriers entrapped with Centella asiatica extract Amanda Gomes Marcelino Perez1 · Jocasta Mileski Machado1 · Kelly Cristina Manhani2 · Patrícia Leo2 · Peky Noriega3 · Maria Helena Ambrosio Zanin2 Received: 3 August 2020 / Accepted: 13 October 2020 © Springer Nature Switzerland AG 2020
Abstract Nanoparticle technology plays an important role in loading active ingredients and being able to increase their stability and performance. In this work, Centella asiatica extract, a plant with known dermatological and cosmetic applications, was incorporated into colloidal polymer nanocarriers, dispersed in silicophilic medium. Nanocarriers were prepared and characterized for particle size (Dynamic Light Scattering), morphology (Scanning Electron Microscopy) and entrapment efficiency of madecassoside. Physical and chemical stability of the encapsulated extract was monitored for 60 days of storage under specific conditions (25 and 40 °C), quantifying the content of madecassoside by HPLC and the particle size distribution. In vitro safety assays, including halo diffusion, cell viability and cell transformation assay were evaluated for free and encapsulated extracts. Nanocarriers showed an average diameter around 210 nm (Polidispersity Index—PDI 0.2) and the entrapment efficiency of 97.7%. The concentration of madecassoside remained stable over time, indicating that the nanocarriers proposed here were able to protect madecassoside against degradation. In addition, nanocarriers showed non-cytotoxic effects and did not induce cell transformation. Keywords Centella asiatica extract · Nanoparticles · Polymeric nanocarriers · Madecassoside
1 Introduction Centella asiatica (L.) Urban (C. asiatica), also known as Gotu Kola, is a small perennial plant [1] used in traditional medicine in different parts of the world, sucha as Southeast Asia, India, China and Indonesia. The main constituents responsible for pharmacological activities are the pentacyclic triterpenes (asiatic acid, madecassic acid, asiaticoside, and madecassoside) [2]. C. asiatica has significantly drawn the attention of scientific groups in the recent years due to its broad range of applications, such as in skin diseases [3], wound healing [4], anti-aging and skin moisturizing [5], hepatoprotective [6], anticancer, antibacterial, antifungal, anti-inflammatory [3], neuroprotective [7, 8], antioxidant
[9, 10], antidiabetic [11], cognitive function enhancement [12]. The literature has numerous success studies indicating the cosmetic potential of C asiatica in the treatment of wrinkles and photoaging skin [13, 14], cellulite and striae [15], and as a skin moisturizing [4, 16]. The skin aging is a multi-factorial phenomenon induced by intrinsic (chronological aging) and extrinsic factors (like ultraviolet damage, pollution, harsh weather, and cigarette smoke) [17], which the chronological aging leads to a decrease in the number of fibroblasts and, consequently, the synthesis of collagen and elastin also decreases, resulting in skin wrinkles and loss o
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