Pretreatment with berberine protects against cisplatin-induced renal injury in male Wistar rats
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ORIGINAL ARTICLE
Pretreatment with berberine protects against cisplatin-induced renal injury in male Wistar rats Hesameddin Allameh 1 & Iman Fatemi 2 & Ali Reza Malayeri 3,4 & Ali Nesari 4 & Saeed Mehrzadi 5 & Mehdi Goudarzi 6,7 Received: 25 August 2019 / Accepted: 17 April 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Berberine (BBR), an isoquinoline alkaloid, has been reported to be an antioxidant agent. This study was conducted to investigate the effect of BBR against nephrotoxicity induced by cisplatin (Cis) in male rats. In this experimental study, 28 Wistar male rats were randomly divided into four groups. Rats were pretreated with BBR (100 mg/kg/day, p.o.) for 7 consecutive days and Cis (7.5 mg/kg, i.p.) was administrated on the 7th day, 1 h after the last dose of BBR. Blood samples were collected to determine blood urea nitrogen (BUN) and creatinine (Cr) levels. Malondialdehyde (MDA), glutathione (GSH), protein carbonyl (PC), and nitric oxide (NO) levels and the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and myeloperoxidase (MPO) were assessed in the left renal tissue. Also, the mRNA expression of SOD2 and PGx1 was measured in the left renal tissue. The right kidney was used for histopathological evaluation. Our results revealed that the levels of Cr, BUN, MDA, NO, and PC and the MPO activity increased by Cis administration. Also, we found that Cis decreased renal GSH level and SOD, GPx, and CAT activities. Pretreatment with BBR for 7 consecutive days significantly attenuated the Cis-induced nephrotoxicity via increasing the antioxidant capacity and reducing the oxidative stress indices in the renal tissue. Moreover, the renoprotective effect of BBR was confirmed by the histopathological evaluation of the kidneys. Our results indicated that BBR has produced amelioration in biochemical indices and oxidative stress parameters against Cis-induced nephrotoxicity. Keywords Nephrotoxicity . Cisplatin . Berberine . Antioxidant . Rat
Introduction Hesameddin Allameh and Iman Fatemi contributed equally to this work. * Mehdi Goudarzi [email protected] 1
Department of Persian Medicine, The School of Persian Medicine, Shahid Sadoughi University of Medical Sciences, Ardakan, Yazd, Iran
2
Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
3
Nab’a Al-Hayat Health Research Center, Nab’a Al-Hayat Foundation for Medical Sciences and Health Care, Najaf, Iraq
4
Department of Pharmacology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5
Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
6
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
7
Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Cisplatin (Cis) is an inorganic platinum compound with antineoplastic effects mainly used for the treatment of solid tumors (Bazmandegan et al. 2019). Cis interacts with DNA
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