Promoting enzymatic saccharification of organosolv-pretreated poplar sawdust by saponin-rich tea seed waste
- PDF / 1,100,965 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 102 Downloads / 159 Views
RESEARCH PAPER
Promoting enzymatic saccharification of organosolv‑pretreated poplar sawdust by saponin‑rich tea seed waste Chenhuan Lai1,2 · Chundong Yang1 · Ying Zhao1 · Yuan Jia1 · Liwei Chen1 · Chengfeng Zhou2 · Qiang Yong1,3 Received: 9 April 2020 / Accepted: 1 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Organosolv pretreatment with two ethanol concentrations (25% and 50%, v/v) was performed to improve enzymatic saccharification of poplar sawdust. It was found that lower ethanol concentration (25%, v/v) pretreatment resulted in a higher enzymatic digestibility of poplar (38.1%) due to its higher xylan removal and similar lignin removal ratios, compared to that pretreated with 50% (v/v) ethanol pretreatment (27.5%). However, the residual lignin still exhibited a strong inhibition on enzymatic hydrolysis of organosolv-pretreated poplar (OP). Bio-surfactant preparations including tea saponin (TS), TS crude extract, and tea seed waste were applied in enzymatic hydrolysis of OP, due to their potential ability of reducing enzyme non-productive binding on lignin. Their optimal loadings in enzymatic hydrolysis of OP were optimized, which indicated that adding 0.075 g/g glucan of TS improved the 72-h glucose yield of OP by 48.3%. Moreover, adding TS crude extract and tea seed waste exhibited the better performance than TS for improving enzymatic hydrolysis of OP. It was verified that the presence of protein in TS crude extract and tea seed waste accounted for the higher improvement. More importantly, the directly application of tea seed waste in enzymatic hydrolysis could achieve the similar improvement on enzymatic hydrolysis of OP, where chemosynthetic surfactant (PEG6000) was added. The residual enzyme activities in supernatant of enzymatic hydrolysis were also determined to reveal the changes on enzyme adsorption after adding surfactants. Generally, tea seed waste could be directly applied as an alternative to chemosynthetic surfactants to promote enzymatic hydrolysis of lignocelluloses. Keywords Poplar sawdust · Organosolv pretreatment · Enzymatic saccharification · Tea saponin · Tea seed waste · Enzyme adsorption
Introduction Lignocellulosic biomass, the most abundant renewable resource, has been considered as an alternative to fossil resources for producing fuels, chemicals, and materials in
* Qiang Yong [email protected] 1
Jiangsu Co‑Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People’s Republic of China
2
State Key Laboratory of Bio‑Fibers and Eco‑Textiles, Qingdao University, Qingdao 266000, People’s Republic of China
3
Key Laboratory of Forestry Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People’s Republic of China
decades [1, 2]. Tremendous efforts have been devoted to biorefinery of lignocellulosic biomass. Compared to herbaceous biomass, woody biomass has been less extensively investigate
Data Loading...
