Supercritical Carbon Dioxide Extraction of Sinensetin, Isosinensetin, and Rosmarinic Acid from Orthosiphon stamineus Lea
- PDF / 2,068,229 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 77 Downloads / 196 Views
RESEARCH ARTICLE-CHEMICAL ENGINEERING
Supercritical Carbon Dioxide Extraction of Sinensetin, Isosinensetin, and Rosmarinic Acid from Orthosiphon stamineus Leaves: Optimization and Modeling Ahmad Hazim Abdul Aziz1,2 · Nicky Rahmana Putra1,2 · Helen Kong2 · Mohd Azizi Che Yunus2 Received: 25 August 2019 / Accepted: 26 April 2020 © King Fahd University of Petroleum & Minerals 2020
Abstract Due to the health benefits such as anti-diabetic, antioxidant, and anti-inflammatory agents, the extraction of bioactive compounds from natural plants becomes significant. One of the favored extraction processes is supercritical carbon dioxide (SC-CO2). SC-CO2 is known as a green method with a high-selectivity process. Therefore, this study aims to determine the best parameters and mass transfer process to extract the bioactive compounds, viz. sinensetin, isosinensetin, and rosmarinic acid, in the leaves of Orthosiphon stamineus using the SC-CO2 extraction technique. Response surface methodology was applied to optimize the operating temperature and pressure of 40–80 °C and 10–30 MPa, respectively. Meanwhile, to quantify the concentration of sinensetin, isosinensetin, and rosmarinic acid, a high-performance liquid chromatography was used. Results showed that the level of sinensetin, isosinensetin, and rosmarinic acid varied significantly at each extraction condition from 122.98 to 469.54 mg/kg sample, 50.60 to 419.07 mg/kg sample, and 17.43 to 931.44 mg/kg, respectively. Based on the optimization study, the optimum pressure and temperature were 10 MPa and 80 °C, respectively, with 440.3 mg/g sinensetin, 392.9 mg/g isosinensetin, and 752.0 mg/g of rosmarinic acid concentration obtained. Meanwhile, the singlesphere model showed a significant correlation with sinensetin, isosinensetin, and rosmarinic acid extraction, with an average AARD of 12.31%, 13.72%, and 18.90%, respectively. The range of diffusivity coefficient, De, of sinensetin, isosinensetin, and rosmarinic acid was 5.47E−13–1.81E−12 m2/s, 8.26E−13–1.89E−12 m2/s, and 2.64E−13–2.28E−12 m2/s, respectively. Keywords Supercritical carbon dioxide · Orthosiphon stamineus · Optimization · Diffusivity · Sinensetin · Isosinensetin · Rosmarinic acid
1 Introduction Orthosiphon stamineus (O. stamineus) is a genus of Lamiaceae and a popular traditional herbal medicine among Asians. It can treat various diseases, including bacterial infections, influenza, and angiogenesis-related problems * Ahmad Hazim Abdul Aziz [email protected] * Mohd Azizi Che Yunus [email protected] 1
Centre of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
School of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
2
such as cancer. Few studies have been conducted on the medicinal properties of O. stamineus. O. stamineus is proven to act as an anti-diabetic [1, 2], anti-obesity [3, 4], antibacterial [5], antifunga
Data Loading...
