Synthesis and Characterization of Antimicrobial Silver Nanoparticles by an Endophytic Fungus Isolated from Nyctanthes ar
- PDF / 450,162 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 77 Downloads / 211 Views
RESEARCH ARTICLE
Synthesis and Characterization of Antimicrobial Silver Nanoparticles by an Endophytic Fungus Isolated from Nyctanthes arbor-tristis Surendra Kumar Gond1 • Ashish Mishra2 • Satish Kumar Verma2 Vijay Kumar Sharma2 • Ravindra Nath Kharwar2
•
Received: 28 May 2019 / Revised: 18 July 2019 / Accepted: 20 August 2019 The National Academy of Sciences, India 2019
Abstract Biofabrication of metal nanoparticles is a costeffective, one-step and ecofriendly technique. Cell filtrate of an endophytic fungus isolated from Nyctanthes arbortristis was challenged with 1 mM solution of AgNO3 for the silver nanoparticles (AgNPs) synthesis. A reduction of silver ions into AgNPs was observed by surface plasmon resonance at absorption maxima 422 nm. The average size of AgNPs was 35.05 nm. The maximum inhibition zones by AgNPs were 14 mm each against E. coli and Pseudomonas aeruginosa compared to AgNO3 solution used as control (10 mm and 9 mm). The fungus was identified as Phomopsis helianthi by the sequencing of ITS region of rDNA. This experiment demonstrates a single-step and ecofriendly method for biosynthesis of AgNPs and their usage as an antimicrobial agent.
Introduction
Significance statement Green synthesis of nanoparticles using fungal biomass is an eco-friendly and cost-effective process. This study signifies that endophytic fungus Phomopsis helianthi could be a good agent for the synthesis of antimicrobial silver nanoparticles.
Nanotechnology is a science of designing, characterization, production, and application of materials, devices, and systems by controlling the shape and size at the nanometer scale [1]. The exercise in nanotechnology is done for the transformation of materials in the form of nanoparticles, nanoclusters, nanodots, nanotubes, nanofibres, nanowires which reduce the cost and space for the application of those materials. The nanoparticles of gold are used in targeted drug delivery in cancer cells, whereas iron NPs are used for groundwater pollutants. Fabric treatment is done with AgNPs for the removal of microbial contaminations [2]. Interestingly, an excellent antiviral activity against HIV-1 was reported from silver nanoparticles. AgNPs bind to gp120 in a manner that prevents CD4-dependent virion binding, fusion, and infectivity, acting as an effective viricidal agent against the cell-free and cell-associated virus [3]. Despite other physical and chemical techniques, using a fungal endophyte Phomopsis helianthi, the authors report the one-step, ecofriendly, and cost-effective biosynthesis of AgNPs and their antibacterial potential. This endophytic fungus resides inside healthy plant tissues of Nyctanthes arbor-tristis without causing any symptoms to the host [4]. Interestingly, fungal endophytes are reported to produce a variety of bioactive and anticancer compounds [5].
& Surendra Kumar Gond [email protected]
Material and Methods
Keywords Endophyte Fungi Nanoparticle Antimicrobial Plasmon resonance
1
2
Department of Botany, MMV, Banaras Hindu University, Varanasi
Data Loading...
