Transcriptional Regulation Methods and Protocols
Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin
- PDF / 10,571,988 Bytes
- 622 Pages / 504.57 x 720 pts Page_size
- 43 Downloads / 193 Views
IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Transcriptional Regulation Methods and Protocols Edited by
Ales Vancura Department of Biological Sciences, St. John’s University, Queens, NY, USA
Editor Ales Vancura, Ph.D Department of Biological Sciences St. John’s University Queens, NY, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-375-2 e-ISBN 978-1-61779-376-9 DOI 10.1007/978-1-61779-376-9 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011941586 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface In the last 20 years, the methodologies used in the field of transcriptional regulation have undergone remarkable changes. Perhaps the most significant advancements are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein–DNA interactions at any genomic loci. The combination of genome-wide approaches and chromatin immunoprecipitation culminated in developing methods for measuring protein–DNA interactions at a genome-wide scale, such as ChIP–chip and ChIP–Seq. The regulatory role of chromatin and histone modifications also came to the forefront during the last 20 years, adding new layers of complexity to our understanding of transcriptional regulation. In addition, mass spectrometry methods identified an array of accessory proteins involved in all stages of RNA synthesis and its regulation. This volume is divided in four parts: (1) Promoter elements, transcription factors, and preinitiation complex (PIC) assembly, (2) Chromatin structure, (3) Chromatin modifying complexes, and (4) RNA synthesis and regulation. Strict classification of the described methods into these four parts, however, is very difficult. For example, ChIP methods are used for studies of transcription factors binding to promoter elements as well as for the characterization of chromatin structure and histone modifications. Most of the protocols presented in this volume hav
Data Loading...
