Tunable plasmon-assisted electrochemiluminescence strategy for determination of the rapidly accelerated fibrosarcoma B-t
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ORIGINAL PAPER
Tunable plasmon-assisted electrochemiluminescence strategy for determination of the rapidly accelerated fibrosarcoma B-type (BRAF) gene using concave gold nanocubes Qian Zhang 1,2 & Zihui Liang 1 & Yixin Nie 1 & Xin Zhang 1 & Qiang Ma 1 Received: 25 August 2020 / Accepted: 29 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A tunable plasmon-assisted electrochemiluminescence (ECL) strategy is reported using concave Au nanocubes (Au CBs) for rapidly accelerated fibrosarcoma B-type (BRAF) detection. Concave Au CBs exhibit a strong surface plasmon coupling (SPC) effect between its sharp apexes and edges. The high spectral overlap with graphite phase carbon nitride quantum dots (g-C3N4 QDs) is achieved by tuning surface plasmon absorption peak of the concave Au CBs. It maximizes the SPC effect and enhances the ECL signal of g-C3N4 QDs 3-fold. The SPC effect of Au CBs is twice as high as with Au NPs. We also employed a toeholdmediated strand displacement (TMSD) strategy for sensitive target recycling amplification. Under optimal conditions, this sensor can determine BRAF gene from 1 pM to 1 nM with a detection limit of 3.06 × 10−5 nM (S/N = 3) and RSD 3.67%. With the aid of the TMSD strategy and tunable plasmon-assisted ECL sensing mode, this sensor also exhibits good analytical performance in human serum with satisfactory recovery of 90.2~109%. The proposed strategy provides a promising method to effectively enhance spectral overlap and detect BRAF gene. Keywords Concave Au cubes . Surface plasmon coupling effect . G-C3N4 QDs . Toehold-mediated strand displacement reactions . Nucleic acid detection . Tunable plasmon-assisted ECL sensing mode
Introduction Plasmonic materials are widely used in Raman scattering, fluorescence, and electrochemiluminescence (ECL), due to their unique surface plasmons and optical properties [1–5]. Noble metal Au nanoparticles (NPs) are the most commonly used surface plasmonic material [6, 7]. For instance, Kooshki and his workers constructed ECL immunosensor with graphene oxide-AuNPs, which improved the ECL intensity of CdS nanocrystals and the detection sensitivity of protein [8]. As is well known, the surface plasmon resonance effect of Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04584-1) contains supplementary material, which is available to authorized users. * Qiang Ma [email protected] 1
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China
2
Electron Microscopy Center, Jilin University, Changchun 130012, China
spherical Au NPs is limited. Moreover, the plasmon-enhanced ECL sensing mode strongly depends on the distance and spectral overlap between the luminophore and the plasmon enhancer [4, 9, 10]. The appropriate distance can be easily achieved with the aid of silica layers [11, 12], or DNA structure [13–15]. However, it is difficult to achieve a high spectral overlap between the luminophore and the plasmon enhancer. On the o
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