Utility of Silane-Modified Magnesium-Based Magnetic Nanoparticles for Efficient Immobilization of Bacillus thermoamylovo
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Utility of Silane-Modified Magnesium-Based Magnetic Nanoparticles for Efficient Immobilization of Bacillus thermoamylovorans Lipase Shikha Rana 1 & Abhishek Sharma 2 & Arun Kumar 1 & Shamsher Singh Kanwar 2 & Mahavir Singh 1 Received: 25 February 2020 / Accepted: 23 June 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Enzymes and protein’s immobilization on magnetic nano supports is emerging as a promising candidate in the food, medical field, and areas of environmental studies. This work presents a study on purified Bacillus thermoamylovorans lipase (BTL) by utilizing tetraethoxysilane (TEOS)-modified magnesium nano ferrite (MgNF) of 20 nm size. Its structural and morphological studies were investigated by powder X-ray diffractometry, high-resolution transmission electron microscopy, etc. Binding of BTL with MgNF was supported by using Fourier transform infrared spectroscopy. Magnetic behavior was examined by the vibrational sample magnetometer and Mössbauer spectrometer graphs. The enzymatic activity of BTL before and after immobilization was studied at different temperatures and reaction time. As per the Lineweaver-Burk plot, immobilized lipase has more biological affinity for fatty acids in comparison to the free lipase, and Kmax values of immobilized and free BTL were computed as 6.6 and 7.5 mM respectively, with excellent reusability(> 50%) even till 13 consecutive assay runs.
Keywords Magnesium nanoferrite . Silane . Immobilization . Mössbauer . Reusability
Introduction Current studies in biotechnical industry are engrossed on the augmentation of heterogeneous catalyst systems [1] which can be lucidly formulate and revitalized easily from the reaction mixture [2]. A catalytic system located on a solid nano support has an edge of facile separation
* Shikha Rana [email protected]
1
Department of Physics, Himachal Pradesh University, Shimla 171005, India
2
Department of Biotechnology, Himachal Pradesh University, Shimla 171005, India
Applied Biochemistry and Biotechnology
in organic and inorganic reactions [3–6]. Ergo, the heterogeneous catalyst immobilization on the nano substrates provides effortless control in the synthesis process and is a convenient option for biochemical and pharmaceutical applications [7–9]. Enzymes are eminently pervasive nano range biocatalysts which are ordinarily explored due to their phenomenal features such as chemical selectiveness, the acceleration rate, selectivity towards substrate, and regional preference in enzyme facilitate reactions. For direct heterogeneous support and enzyme immobilization, the deformation of active catalytic sites is the common concern of researchers, which generally out turn a loss of activity [9]. In this context, functionalized iron oxide-based recyclable nanomaterial like ferrites [2, 10] are acquiring a lot of considerations due to their exclusive magnetic properties, high exterior surface to volume proportion, low toxicity, and the chemically resistant structure that help in developing an advan
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