Variation of antibody responses to Plasmodium falciparum MSP1-19 antigen with parasitaemia and IL4vntr polymorphism in K
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ORIGINAL ARTICLE
Variation of antibody responses to Plasmodium falciparum MSP119 antigen with parasitaemia and IL4vntr polymorphism in Khartoum state, Sudan Hind M. Abushama1 • Inas A. AbdelRahman1 • Hiba Ali1 • Tasneem Mowia1 • Faisal Mousa1 Muzamil M. Abdelhamid2 • Ibrahim M. ElHassan2
•
Received: 26 April 2020 / Accepted: 3 November 2020 Ó Indian Society for Parasitology 2020
Abstract A hospital-based cross-sectional study was conducted at Khartoum state to investigate the variation of antibody responses to Plasmodium falciparum 19-kDa C-terminal region of merozoite surface protein 1 antigen and the variation of human IL4 polymorphism with parasitaemia. Measurements of natural acquisition of antiPlasmodium falciparum MSP1-19 IgG, IgG1 and IgG3 antibodies were performed using ELISA. Molecular characterization of IL4vntr polymorphism was achieved. We were able to detect a statistically significant negative correlation between parasitaemia and different age groups (r = - 0.262 and p value = 0.043) and with anti-P.fMSP119 IgG1 (r = - 0.418, p value = 0.047). Anti-P.fMSP1-19 IgG showed a significant difference among age groups (p \ 0.001). Only anti-P.fMSP1-19 IgG showed a & Hind M. Abushama [email protected] Inas A. AbdelRahman [email protected] Hiba Ali [email protected] Tasneem Mowia [email protected] Faisal Mousa [email protected] Muzamil M. Abdelhamid [email protected] Ibrahim M. ElHassan [email protected] 1
Department of Zoology, Faculty of Science, University of Khartoum, Khartoum, Sudan
2
Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan
significant association with general appearance (p value \ 0.001). The mean for total anti-P.fMSP1-19 IgG3 was statistically significantly higher in females compared to males (p value \ 0.001). There was no significant difference in the distribution of human IL4 vntr genotypic and allelic frequencies between cases and control group as well as among different clinical manifestation.We concluded that IgG1 levels to MSP1-19 were found to be negatively correlated with parasitaemia and anti-PfMSP1-19 IgG was significantly increased in ill and severely ill with age considered as a cofactor. Further studies are needed to ascertain the role of IgG and IgG1 in protection and to investigate the IgG and subclasses’ response against other antigenic markers. These findings are valuable for advancing vaccine development by providing evidence supporting merozoite antigens as targets of protective immunity in humans. Keywords Plasmodium falciparum MSP1-19 IgG IgG1 IgG3 IL4vntr Parasitaemia Sudan
Background A substantial decline of Plasmodium falciparum infection has been witnessed in Sudan in the past few years following the implementation of new control strategies, even though malaria is still considered as the major cause of febrile illness in the country. Symptomatic malaria accounts for 20–40% of outpatient clinic visits and approximately 30% of hospital admissions. More than hundred thousand severe malaria cas
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