Vertebrate Embryogenesis Embryological, Cellular, and Genetic Method
This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional mo
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Francisco J. Pelegri Editor
Vertebrate Embryogenesis Embryological, Cellular, and Genetic Methods Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Vertebrate Embryogenesis Embryological, Cellular, and Genetic Methods Second Edition
Edited by
Francisco J. Pelegri Laboratory of Genetics, University of Wisconsin–Madison, Madison, WI, USA
Editor Francisco J. Pelegri Laboratory of Genetics University of Wisconsin–Madison Madison, WI, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9008-5 ISBN 978-1-4939-9009-2 (eBook) https://doi.org/10.1007/978-1-4939-9009-2 Library of Congress Control Number: 2018966818 © Springer Science+Business Media, LLC, part of Springer Nature 2019 Chapter 10 is licensed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/). For further details see license information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Despite continuous and fast-paced progress, much remains unknown in our understanding of biological processes. No exception is the field of animal embryogenesis, even as this field was one of the earliest to gain attention with detailed genetic, cellular, and molecular analyses. This is particularly true for vertebrate species, due to their relative intractability compared to invertebrates. Yet vertebrate systems, and their earliest stages of development, continue to both capture the attentio
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