PCR Methods and Protocols
This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and syntheti
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Lucília Domingues Editor
PCR
Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
PCR Methods and Protocols
Edited by
Lucília Domingues CEB—Centre of Biological Engineering, University of Minho, Braga, Portugal
Editor Lucília Domingues CEB—Centre of Biological Engineering University of Minho Braga, Portugal
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7059-9 ISBN 978-1-4939-7060-5 (eBook) DOI 10.1007/978-1-4939-7060-5 Library of Congress Control Number: 2017940257 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Discovered by Kary Mullis in 1983, the polymerase chain reaction (PCR) is a breakthrough technology that has allowed the advancement of different scientific fields, being a fundamental tool in current scientific research. As such, significant literature exists on the basics of the PCR technique, but the specificities of its application to different areas of the biotechnology and bioengineering field is mostly dispersed. Despite being a well-established technique, novel applications are constantly emerging given the power and flexibility of PCR, with continuous updates being published in this very exciting and moving field. Thus, along with cutting-edge methodologies, this volume focuses on many core PCR applications in the biotechnology and bioengineering field. In the initial chapters, software for in silico PCR and primer design as
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