Cell Cycle Synchronization Methods and Protocols
To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing
- PDF / 412,228 Bytes
- 12 Pages / 504 x 720 pts Page_size
- 61 Downloads / 235 Views
IN
MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
TM
Cell Cycle Synchronization Methods and Protocols
Edited by
Gaspar Banfalvi Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary
Editor Gaspar Banfalvi Department of Microbial Biotechnology and Cell Biology University of Debrecen Debrecen 4010, Hungary [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-181-9 e-ISBN 978-1-61779-182-6 DOI 10.1007/978-1-61779-182-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011931108 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface What Kind of Cells Can Be Synchronized? To study how cells progress through the cell cycle, cell cultures have to be brought to the same phase. The unique feature of this book is exactly this: to prepare synchronized cells representing different stages of the cell cycle. The book also shows the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. The synchronization methods presented in the book are based principally on two major strategies. The “arrest-and-release” approach involves different chemical treatments to block cells at certain stages of the cell cycle. The physical strategy contains physical methods to collect cells belonging to subpopulations of the cell cycle. The collection of synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures consisting of individual cells is described by professionals of their respective field. Additional chapters include synchronization of transfected and embryonic cells.
Why Edit a Book on Synchronization? This question can be easily answered by making a computer search typing in the words “cell synchronization.” It turns out that there are many scientists who are desperately looking for synchronization protocols. Researchers are interested in synchronizing mammalian, plant, yeast, fungal, and even bacterial cells but do not know how to do i
Data Loading...
