Response Surface Methodology Optimization Extraction of Polysaccharides from Maca (Lepidium meyenii) Leaves and Primary
Most of studies have focused on the maca (Lepidium Meyenii) hypocotyls, including the extraction, purification, characterization of bioactive components and their structures, but few on maca leaves. In this paper, we used response surface methodology to o
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1 Introduction Response surface methodology (RSM) has proved quite an effective method for the expected purpose. Recently, RSM has been widely used to optimize processes in many studies, including the extraction of polysaccharides. Box-Behnken design (BBD), the most impotent and common design of RSM, is helpful to design experiments, build models, evaluate the effects of factors and searching optimum condition of factors for desirable responses [1]. At present, the RSM has been successfully applied to extract Bletillastriata polysaccharides [2]. Maca (Lepidium meyenii), a medicine food homology plant, belongs to the Brassicaceae family [2]. Maca roots (hypocotyls), the subterranean part, have been a traditional medicinal agent and dietary staple since pre-Columbian times [3]. Maca roots are consumed worldwide as liquor, pills, soft drinks and candies for its high nutritional value and multi-functional properties [4]. Reported properties C. Kang L. Zhang (&) L. Hao (&) H. Ge M. Xu J. Cao Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China e-mail: [email protected] L. Hao e-mail: [email protected] L. Hao J. Yu The Quartermaster Equipment Institute of General Logistics, Department of People’s Liberation Army, Beijing 100010, People’s Republic of China Y. Yang Lijiang Maca Biological Technology Co., Ltd., Kunming 674119, Yunnan, People’s Republic of China © Springer Nature Singapore Pte Ltd. 2018 H. Liu et al. (eds.), Advances in Applied Biotechnology, Lecture Notes in Electrical Engineering 444, https://doi.org/10.1007/978-981-10-4801-2_22
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include energizing, fertility-enhancing effects, antifatigue [5], anti-inflammation [6], antiosteoporosis [7], antidepression [8], antioxidant activity [9], and so on. However, little information has been published on maca leaves, such as polysaccharides. Polysaccharides are macromolecules composed of more than ten monosaccharide units. And the monosaccharides are linked by glycosidic bonds. Plant polysaccharides have been used in the food industry and in medicine for a long time. They have attracted excessive attention in recent years [10] because of their exhibited biocompatibility, low toxicity and pharmaceutical biomedical activity, such as antioxidant, immunomodulation, anti-tumor, anti-virus, anticomplementary, and anti-inflammatory activities [11]. Therefore, this paper optimized the extraction of maca leaves polysaccharide by RSM. In addition, the primary characterization of the crude MLPs were described, which may improve some theory value for the further study and comprehensive utilization of maca leaves.
2 Materials and Methods 2.1
Material
Fresh maca leaves were collected from Yunnan Province during the months of November-December, 2013, and identified by Professor Wenbin Hou, Tianjin Institute of Pharmaceutical Research, China. The leaves were shade dried, powdered, sieved through 800 lm and stored at room temperature
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