Sensitive analysis of doxorubicin and curcumin by micellar electromagnetic chromatography with a double wavelength excit
- PDF / 534,964 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 55 Downloads / 185 Views
RESEARCH PAPER
Sensitive analysis of doxorubicin and curcumin by micellar electromagnetic chromatography with a double wavelength excitation source Xiao Yu 1 & Wanxiang Yu 1 & Xiufen Han 1 & Zuanguang Chen 2 & Shumei Wang 1,3 & Haiyun Zhai 1 Received: 17 August 2020 / Revised: 6 October 2020 / Accepted: 16 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Doxorubicin has been extensively used to treat cancers, and there are recent findings that the anticancer activities can be enhanced by curcumin. Although the two compounds have native fluorescence, they can hardly be quantified directly simultaneously using the laser-induced fluorescence (LIF) detection method. To avoid complex fluorescence derivatization and introduction of interfering components, a highly sensitive double wavelength excitation source LIF (D-W-Ex-LIF) detector composed of a 445-nm and 488-nm commercial laser diode was constructed to detect them simultaneously. Rhodamine 6G was selected as an internal standard, because its fluorescence can be excited at 445 nm and 488 nm. The native fluorescence of doxorubicin and curcumin and their resolution were enhanced by introducing mixed micelles. The optimal electrophoretic separation buffer was 10 mM borate buffer containing 20 mM Triton X-100, 5 mM sodium dodecyl sulfate, and 30% (v/v) methanol at pH 9.00. Therefore, the developed method was specific, accurate, and easily operable. Its limits of detection for doxorubicin and curcumin in human urine samples were 4.00 × 10−3 and 1.00 × 10−2 μg/mL, respectively, and the limits of quantification were 1.00 × 10−2 and 3.00 × 10−2 μg/mL, respectively. The recoveries were 94.9–109.1%. Keywords Double wavelength excitation source . LIF detector . Doxorubicin . Curcumin . Micelle electrophoresis
Introduction Doxorubicin, as one of the most effective anthracyclines [1, 2], is usually used as a first-line drug to treat cancers [3]. Nevertheless, its applications in chemotherapy are limited due to severe adverse reactions [4]. Thus, developing a strategy for the rapid determination of doxorubicin is of great significance to clinical treatment. As a spice and traditional herbal medicine found in turmeric [5], curcumin has
* Xiao Yu [email protected] * Haiyun Zhai [email protected] 1
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
2
School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China
3
Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China
pharmacological, antibacterial, antifibrotic, antidepressant, antioxidative, antiviral, anticancer, and anti-inflammatory activities [6–9]. Recently, it has been found that curcumin not only enhanced the anticancer activities of doxorubicin but also relieved the concomitant marrow suppression, cardiac toxicity, oral ulcer, and other side effects of doxorubicin [10–13]. Therefore, developing a highly sensitive method to q
Data Loading...
