Single Cell Protein Analysis Methods and Protocols
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of
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Anup K. Singh Aarthi Chandrasekaran Editors
Single Cell Protein Analysis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Single Cell Protein Analysis Methods and Protocols
Edited by
Anup K. Singh and Aarthi Chandrasekaran Sandia National Laboratories, Livermore, CA, USA Joint BioEnergy Institute, Emeryville, CA, USA
Editors Anup K. Singh Sandia National Laboratories Livermore, CA, USA
Aarthi Chandrasekaran Sandia National Laboratories Livermore, CA, USA
Joint BioEnergy Institute Emeryville, CA, USA
Joint BioEnergy Institute Emeryville, CA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2986-3 ISBN 978-1-4939-2987-0 (eBook) DOI 10.1007/978-1-4939-2987-0 Library of Congress Control Number: 2015952453 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Proteins are one of the most important classes of molecules in living cells and tissues. Proteins and their complexes are involved in virtually all cellular processes including catalysis of biochemical reactions, transport of molecules across membranes, cell growth and division, cell adhesion and migration, and providing structural support. Characterizing the quantity, association, and activity of proteins is therefore critical for understanding the molecular mechanisms of cellular processes involved in cell differentiation and fate, cell signaling, disease progression, and for discovery and development of novel therapeutics, vaccines, and diagnostics. Measuring DNA and RNA can provide qualitative information on gene
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