SlHyPRP1 and DEA1 , the multiple stress responsive eight-cysteine motif family genes of tomato ( Solanum lycopersicum L.
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RESEARCH ARTICLE
SlHyPRP1 and DEA1, the multiple stress responsive eight-cysteine motif family genes of tomato (Solanum lycopersicum L.) are expressed tissue specifically, localize and interact at cytoplasm and plasma membrane in vivo Banashree Saikia1,2 • Johni Debbarma1,2 • Jitendra Maharana3,4 • Dhanawantari L. Singha1 • Natarajan Velmuruagan5 • Hariprasanna Dekaboruah 1,2 6 1,2 • Kallare P. Arunkumar • Channakeshavaiah Chikkaputtaiah Received: 27 August 2020 / Revised: 22 October 2020 / Accepted: 17 November 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Owing to rapid global climate change, the occurrence of multiple abiotic stresses is known to influence the outburst of biotic stress factors which affects crop productivity. Therefore, it is essential to understand the molecular and cell biology of key genes associated with multiple stress responses in crop plants. SlHyPRP1 and DEA1, the members of eight-cysteine motif (8CM) family genes have been recently identified as putative regulators of multiple stress responses in tomato (Solanum Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00913-z) contains supplementary material, which is available to authorized users. & Channakeshavaiah Chikkaputtaiah [email protected]; http://www.rrljorhat.res.in/1261.php
lycopersicum L.). In order to gain deeper insight into cell and molecular biology of SlHyPRP1 and DEA1, we performed their expression analysis in three tomato cultivars and in vivo cell biological analysis. The semi-quantitative PCR and qRT-PCR results showed the higher expression of SlHyPRP1 and DEA1 in leaf, stem, flower and root tissues as compared to fruit and seed tissues in all three cultivars. The expression levels of SlHyPRP1 and DEA1 were found to be relatively higher in a wilt susceptible tomato cultivar (Arka Vikas) than a multiple disease resistant cultivar (Arka Abhed). In vivo cell biological analysis through Gateway cloning and Bi-FC assay revealed the 2
Academy of Scientific and Innovative Research (AcSIR), Uttar Pradesh, Ghaziabad 201 002, India
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Distributed Information Centre (DIC), Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam, India
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Present Address: Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan
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Biological Sciences Division, Branch Laboratory-Itanagar, CSIR-NEIST, Arunachal Pradesh, Naharlagun 791 110, India
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Central Muga Eri Research and Training Institute (CMER&TI), Lahdoigarh, Jorhat, Assam 785 700, India
Banashree Saikia [email protected] Johni Debbarma [email protected] Jitendra Maharana [email protected] Dhanawantari L. Singha [email protected] Natarajan Velmuruagan [email protected] Hariprasanna Dekaboruah [email protected] Kallare P. Arunkumar [email protected] 1
Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology (CSIR-NEIST), Jorhat, Assam 785 006, India
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