Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Reso
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Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Resonance Immunosensor Mingfei Pan 1 & Xiaojun Wang 1 & Junping Wang 1 & Yang Lu 1 & Kun Qian 1 & Shuo Wang 1
Received: 11 October 2016 / Accepted: 6 December 2016 # Springer Science+Business Media New York 2016
Abstract In this research, a reproducible surface plasmon resonance (SPR) immunosensor based on inhibition format was fabricated for stable and sensitive detection of four sulfonamides (SAs) in animal-derived foods. The parameters in the fabrication and measurement process were optimized and discussed in details. The method using the proposed SPR immunosensor was validated and exhibited favorable performance for SAs residues detection in common animal-derived food products, as well as acceptable accuracy (pure milk 88.5–106.2%; egg 89.0–91.5%; chicken muscle 87.2–94.7%; beef 84.8–96.9%; fish 87.8– 95.0%), precision (relative standard deviation (n = 3), pure milk 3.2–5.2%; egg 1.1–5.4%; chicken muscle 1.1–5.9%; beef 2.5– 7.1%; fish 4.6–5.3%), and sensitivity (IC 15 , pure milk 59.6 ng mL − 1 , egg 56.1 ng mL − 1 , chicken muscle 66.9 ng mL−1, beef 63.3 ng mL−1, fish 62.7 ng mL−1). Each detection cycle could finish in less than 5 min, and each SPR chip could reuse 300 analysis cycles with response attenuation of 3.7%. These results have demonstrated that the proposed SPR immunosensor has offered an effective, accurate, sensitive, rapid, and low-cost methodology for SAs residue detection in food sample, and this method has great potential for the routine analysis of large numbers of samples on measuring different kinds of compounds.
Electronic supplementary material The online version of this article (doi:10.1007/s12161-016-0752-7) contains supplementary material, which is available to authorized users. * Shuo Wang [email protected] 1
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China
Keywords Sulfonamides . Surface plasmon resonance . Immunosensor . Animal-derived foods
Introduction In recent years, food safety has attracted more attention from society and governments all over the world. For animalderived foods, an important source of its insecurity is the residue of veterinary drugs, which seriously affects the quality of food and can threaten to human health (Baynes et al. 2016; Frenich et al. 2014). Sulfonamides (SAs) are one of the most widely administered types of antibiotics that are used not only for the treatment of bacterial infections but also for prophylactic purposes in animal production because of their cost effectiveness and wide-spectrum antimicrobial activity (Campbell. 2008; Ceruso et al. 2014). This class of drugs was potentially hazardous to human health including sensitive humans, immune system damage, and formation of bacterial resistance. To protect humans from the risks related to SA residues, strict regulations have been legally established in several countries and organiz
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