Stem Cells for Myocardial Regeneration Methods and Protocols
There is currently a great deal of enthusiasm surrounding organ regeneration, which mainly comes from the potential pleuripotency of stem cells to differentiate into various tissue types. In Stem Cells for Myocardial Regeneration: Methods and Protocols, e
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Stem Cells for Myocardial Regeneration Methods and Protocols
Edited by
Randall J. Lee Department of Medicine, Cardiovascular Research Institute, and Institute of Regeneration Medicine, University of California San Francisco, San Francisco, CA, USA
Editor Randall J. Lee, MD, Ph.D. Department of Medicine, Cardiovascular Research Institute, and Institute of Regeneration Medicine, University of California San Francisco, San Francisco, CA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-704-4 e-ISBN 978-1-60761-705-1 DOI 10.1007/978-1-60761-705-1 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010931703 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana press is a part of Springer Science+Business Media (www.springer.com)
Preface The field of regenerative medicine is in its infancy state. Enthusiasm for the potential of organ regeneration lies with the potential pluripotency of stem cells to differentiate into various tissue types. This volume of Methods in Molecular Biology will focus on the use of stem cells for myocardial repair and regeneration. The emphasis of this issue will be to provide basic scientists, translational investigators, and cardiologists a means to evaluate the efficacy and safety of stem cells in a standardized fashion for myocardial regeneration. Many different cell types have been considered for myocardial repair. Adult cardiomyocytes are unable to survive even when transplanted into normal myocardium. The use of fetal or neonatal cardiomyocytes is not a feasible source of cells due to ethical concerns and donor availability. Therefore, the use of pluripotent stem cells has become the focus of a cell source
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