Synthesis of silver and gold nanoparticles by Sargassum muticum biomolecules and evaluation of their antioxidant activit
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ORIGINAL RESEARCH
Synthesis of silver and gold nanoparticles by Sargassum muticum biomolecules and evaluation of their antioxidant activity and antibacterial properties N. González‑Ballesteros1 · M. C. Rodríguez‑Argüelles1 · M. Lastra‑Valdor2 · G. González‑Mediero3 · S. Rey‑Cao3 · M. Grimaldi4 · A. Cavazza4 · F. Bigi4,5 Received: 24 July 2020 / Accepted: 13 September 2020 © Islamic Azad University 2020
Abstract Abstract The present work reports on the antibacterial and antioxidant activity of gold and silver nanoparticles, synthesized by an extract of the brown macroalga Sargassum muticum (SM). These nanoparticles were synthesized and fully characterized by means of UV–Vis spectroscopy and electron microscopy. These techniques confirmed that spherical nanoparticles, with mean diameters of 10.4 ± 1.2 nm for Au@SM and 41.0 ± 5.7 nm for Ag@SM, were formed. To investigate the possible role of these biomolecules in this green synthetic process, Fourier-transformed infrared spectroscopy analysis was performed before and after the synthesis of nanoparticles. Furthermore, the carbohydrate composition was examined along with other variations observed after the synthesis of nanoparticles by size-exclusion chromatography. The study of the changes observed indicated that the polysaccharide fraction of the extract plays a role in the formation of the nanoparticles as well as in their stabilization. The in vitro antioxidant activity was analyzed by the determination of the reducing power, total content of phenolic compounds and the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging activity. Lastly, the antibacterial activity was tested against three types of bacteria with different cell wall composition. In particular, Ag@SM showed good inhibitory capacity on Gram+, especially on Staphylococcus aureus with a MIC of 3.38 µg/mL. Graphic abstract
Keywords Sargassum muticum · Gold nanoparticles · Silver nanoparticles · Antibacterial activity · Antioxidant activity * M. C. Rodríguez‑Argüelles [email protected] Extended author information available on the last page of the article
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Vol.:(0123456789)
Journal of Nanostructure in Chemistry
Introduction Despite the numerous existing antibiotics and other antimicrobial drugs, bacterial infections are still one of the most significant causes of morbidity all over the world. The growing concern about multidrug-resistant bacterial strains [1] makes it necessary to develop agents with potent antibacterial power. The increase in infections caused by microbes, the rapid development of resistance to present-day antibiotics, and the fast evolution of bacteria due to mutation are factors that account for the need to develop or modify compounds with an antibacterial action, as well as other treatments. Cutting-edge research in nanotechnology has led, in recent times, to the development of nanoscopic materials with significant antibacterial action against multidrug-resistant pathogens which could, hence, constitute an excellent platform to fight bacterial mutation [2]. It has been demons
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