Comparative evaluation of silver nanoparticles biosynthesis by two cold-tolerant Streptomyces strains and their biologic
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ORIGINAL RESEARCH PAPER
Comparative evaluation of silver nanoparticles biosynthesis by two cold-tolerant Streptomyces strains and their biological activities Ali Bakhtiari-Sardari . Mansour Mashreghi . Hossein Eshghi . Fatemeh Behnam-Rasouli . Elham Lashani . Bahar Shahnavaz
Received: 8 January 2020 / Accepted: 20 May 2020 Ó Springer Nature B.V. 2020
Abstract The present study reflected on high-priority biological activities of novel silver nanoparticles (AgNPs) synthesized via two cold-tolerant strains; namely, Streptomyces sp.OSIP1 and Streptomyces sp.OSNP14. These AgNPs were synthesized through a green method using culture supernatant of bacteria at 20 °C and characterized by several instrumental techniques. The TEM results revealed that the NPs obtained from OSIP1 were smaller (8 nm, average) than those taken from OSIP14 (15 nm, average). Both AgNPs-OSP1 and AgNPs-OSNP14 also posed the strongest growth inhibitory effect against several pathogenic bacteria alone and especially in combination with antibiotics. Smaller NPs especially at 3.9–31.25 lg/ml concentrations were assumed more A. Bakhtiari-Sardari M. Mashreghi E. Lashani B. Shahnavaz (&) Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran e-mail: [email protected] M. Mashreghi F. Behnam-Rasouli Novel Diagnostic and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, 9177948974 Mashhad, Iran H. Eshghi Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran B. Shahnavaz Institute of Applied Zoology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
effective biofilm inhibitors of Pseudomonas aeruginosa. Cytotoxic activity of both AgNPs (at 25 and 50 lg/mL concentrations) on mouse colorectal carcinoma cells (CT26) were then studied using methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The findings demonstrated that smaller AgNPs at a 50 lg/mL concentration had 7% more cytotoxicity effects. In conclusion; although AgNPs produced by diverse strains of cold-adapted Streptomyces had close characteristics and biological activities, they showed some multifarious properties. Keywords Cold-tolerant AgNPs Antimicrobial activity Synergism CT26 cell cytotoxicity
Introduction Silver nanoparticles (AgNPs) are distinguished as silver structures of less than 100 nm in size, which are increasingly used in medical industries on account of their unique physical, chemical, and biological properties (Mude et al. 2009). Such AgNPs can, however, damage and eventually destroy bacteria cells through various mechanisms including interaction with cell membranes, interaction with deoxyribonucleic acids (DNAs), deactivation of enzymes, as well as changes in protein expressions (Lok et al. 2006). These NPs are known to have antimicrobial effects against various
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human and animal pathogens (Taglietti et al. 2012). Many studies have focused on the effects of AgNPs on
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