The mitochondrial genome of a stonefly species, Cerconychia sapa (Plecoptera: Styloperlidae)
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TECHNICAL NOTE
The mitochondrial genome of a stonefly species, Cerconychia sapa (Plecoptera: Styloperlidae) Ying Wang1,2 · Jinjun Cao1 · Beibei Lei1 · Weihai Li1
Received: 21 May 2017 / Accepted: 26 May 2017 © Springer Science+Business Media Dordrecht 2017
Abstract The nearly complete sequence of the mitochondrial DNA (mtDNA) of the stonefly, Cerconychia sapa, has been completed and annotated in this study. The circular genome is 15,188 bp in length with an A+T content of 71.2%, and contains 13 PCGs, 22 tRNA genes and 2 rRNA subunit genes. The control region can only be assembled partially. All PCGs use normal start codon ATN, while ND1 and ND5 use TTG and GTG as start codon, respectively. Meanwhile, 12 PCGs use the typical termination codons TAN, except ND5, which stopped with the incomplete terminaton signal T. Phylogenetic analysis suggests that C. sapa is closely related to Styloperla sp. and S. spinicercia. Our results provide basic data for further study of phylogeny in Plecoptera. Keywords Cerconychia sapa · Mitochondrial genome · Phylogenetics
Introduction Styloperlidae, a small stonefly family, is primarily distributed in southern China currently including four Cerconychia Klapálek, 1913 species and six Styloperla Wu, 1935 species. The stonefly C. sapa is the first Cerconychia species distributed both in China and Vietnam; it was described nearly 100 years after its former congeners by Klapálek * Weihai Li [email protected] 1
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, Henan, China
2
Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang, Henan, China
and considered rare in the stonefly groups (Stark and Sivec 2007; Yang and Li 2017). Stoneflies are among the most environmentally sensitive freshwater aquatic fauna (Master et al. 2000). Their potential use as biomonitoring for water quality assessment and the diet of the red-spotted newt is well-known (Stewart and Stark 2008; Stewart et al. 2001; Qian et al. 2014). Unfortunately, habitat destruction and other anthropogenic activities have led to rapid population declines of stoneflies. Therefore, urgent preservation have become necessary for these valuable species. An improved understanding of its genetic diversity would be essential to the formulation of conservation strategies. In this study, we assembled and characterized the mitochondrial genome of C. sapa by high-throughput sequencing method for the first time. The sequence is accessible from GenBank with the accession number MF100783. This study would supply important basis for conservation genetics of C. sapa, and promote the phylogenetic analysis of Plecoptera. The male adult sample of C. sapa was collected from Yakou forestry station of Yunnan Province, China in 2015. The thorax muscle of the specimen was used to extract total genomic DNA. Vouchers consisting of the remaining stoneflies were deposited in the Entomological Museum of Henan institute of Science and Technology (HIST), Henan Province, China. The mitogenomes
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