The complete mitochondrial genome of the toad species Oreolalax jingdongensis (Anura: Megophryidae) and related phylogen
- PDF / 1,824,719 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 21 Downloads / 154 Views
METHODS AND RESOURCES ARTICLE
The complete mitochondrial genome of the toad species Oreolalax jingdongensis (Anura: Megophryidae) and related phylogenetic analyses Liuchenghai Jiang1,2 · Shize Li3 · Le Wang4 · Xinyu Gao1 · Gang Wang1 · Bin Wang1
Received: 20 September 2017 / Accepted: 13 October 2017 © Springer Science+Business Media B.V. 2017
Abstract The complete mitochondrial genome of Oreolalax jingdongensis occurring from the southwestern mountains of China was determined. The new complete mitochondrial genome is 17,864 bp in length and contain 13 protein-coding genes, 2 rRNA genes, 23 tRNA genes, and a control region. Different from the typical gene order in most of anuran species, structure of the mitochondrial genome of O. jingdongensis has a tandem duplication of two tRNA-Met genes and rearrangements of several tRNA genes. Phylogenetic relationships was reconstructed base on the sequences of 13 protein-coding genes from the complete mitochondrial genome of 18 representative Megophryidae species using Bayesian inferences and Maximum likelihood approaches, and the results supported the monophyly of this family. This study supplied basic genetic data for further genetic research and conservation of the toad group. Keywords Megophryidae · Oreolalax jingdongensis · Complete mitochondrial genome · Phylogenetic analyses
* Bin Wang [email protected] 1
CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, The Chinese Academy of Sciences, P. O. Box 416, Chengdu 610041, China
2
High School No. 7 Chengdu, Chengdu 610041, China
3
Maotai University, Zunyi 564500, China
4
Nanjing Institute of Environmental Sciences Under Ministry of Environmental Protection, Nanjing 210000, China
The toad genus Oreolalax Myers and Leviton, 1962 is distributed in the southwestern China and adjacent Vietnam (Frost 2017). Oreolalax jingdongensis is only known from the Ailao Mountains in Yunnan Province of China (Fei et al. 2012). The species was listed as Vulnerable (VU) species (Jiang et al. 2016). The complete mitochondrial genome would promote the future genetic research and conservation of this narrow-distributed VU species. In this study, one toe of one male adult O. jingdongensis was collected from the type locality of the species (Xujiaba, Jingdong County, Yunnan Province, China) and was preserved in 95% ethanol. The total DNA was extracted from the tissue using the DNeasy Tissue Kit (QIAGEN). The DNA fragments were amplified and subjected to polymerase chain reaction (PCR) with twenty pairs of primers as designed by this study. The amplified PCR products were purified using a Qiagen PCR purification kit, and sequences were obtained from an ABI 3500 automated sequencer. Newly determined sequence was deposited in GenBank Accession Number MF953479. The complete mitochondrial genome of O. jingdongensis (Fig. 1) has a total length of 17,864 bp and contains 38 genes, incl
Data Loading...
