Trimer-based aptasensor for simultaneous determination of multiple mycotoxins using SERS and fluorimetry
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ORIGINAL PAPER
Trimer-based aptasensor for simultaneous determination of multiple mycotoxins using SERS and fluorimetry Zhengzong Wu 1 & Deyun He 1 Qingqing Chai 1
&
Bo Cui 1 & Zhengyu Jin 2 & Enbo Xu 3 & Chao Yuan 1 & Pengfei Liu 1 & Yishan Fang 1 &
Received: 17 April 2020 / Accepted: 5 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract An aptasensor is reported for the detection of three different kinds of mycotoxins, i.e., zearalenone (ZEN), ochratoxin A (OTA), and fumonisin B1 (FB1). Based on fluorescence resonance energy transfer effect (FRET) and surfaceenhanced Raman scattering (SERS), the levels of ZEN, FB1, and OTA can be simultaneously determined. Under 980-nm and 650-nm laser excitation, the logarithmic values of fluorescence signal intensities at 543 nm and 670 nm are slowly increased as the concentrations of ZEN and OTA vary from 0.1 ng mL−1 and 0.05 ng mL−1 to 100 ng mL−1 and 25 ng mL−1, respectively. For FB1, under 980-nm laser excitation, the logarithmic value of SERS signal intensity at 1567 cm−1 gradually increases with the concentration of FB1 in the range 0.05–200 pg mL−1 (R2 = 0.996). The detection limits of the proposed assay for ZEN, OTA, and FB1 are 0.03 ng mL−1, 0.01 ng mL−1, and 0.02 pg mL−1, respectively. The selectivity experiment results indicate this assay possesses a high selectivity over other commonly encountered mycotoxins. The average recoveries range from 90 to 107%, revealing satisfactory application potential of the proposed assay. The developed aptasensor will bring bright prospects for research in the field of multiplexed mycotoxine detection.
Keywords Food analysis . Food safety . Cereal products . Nanoprobe . Mycotoxins
Introduction
Zhengzong Wu and Deyun He contributed equally to the work and should be regarded as co-first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04487-1) contains supplementary material, which is available to authorized users. * Deyun He [email protected] * Bo Cui [email protected] 1
State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
2
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
3
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Numerous cases of mycotoxin caused foodborne illness occur in the world annually, causing a great deal of deaths and huge economic losses to both developing countries and developed countries [1–3]. According to the reported studies, overconsumption of mycotoxin-contaminated food and agricultural products may result in side effects for people, including a decreased rate of growth and depression, creation of tumors in some organs like the liver, colon, and kidney, and increased risk of hepatic cancer. [4–7]. As a result, there is an urgent demand for efficient and rapid detection of mycotoxin
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